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A Fully Automated System for Measuring Cellular Mechanical Properties
Wang ZB(王智博); Liu LQ(刘连庆); Wang YC(王越超); Xi N(席宁); Dong ZL(董再励); Li M(李密); Yuan S(袁帅)
Department机器人学研究室
Source PublicationJournal of Laboratory Automation
ISSN2211-0682
2012
Volume17Issue:6Pages:443-448
Indexed BySCI ; EI
EI Accession number20132316393556
WOS IDWOS:000313344200005
Contribution Rank1
KeywordAutomation Mechanical Properties Of Cells Atomic Force Microscopy (Afm) Local Scan
Abstract

As a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping cellular mechanical properties are mostly carried out manually, resulting in measurements that are time-consuming with low efficiency. In this article, a fully automated system of measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, the cells are recognized using an image-processing method and the relative position of the cell, and the AFM tip is accurately calibrated by the local scan method, meaning that the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. In addition, with the implementation of the automation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. The capability of our system is validated on Raji cells, and the results indicate that the measurement rate of our system is 26 times faster than that of the traditional manual method, providing the technology for high-throughput measurement of cellular mechanical properties.

Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS KeywordAtomic-force Microscopy ; Cells ; Afm
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/10330
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Graduate University of Chinese Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States
Recommended Citation
GB/T 7714
Wang ZB,Liu LQ,Wang YC,et al. A Fully Automated System for Measuring Cellular Mechanical Properties[J]. Journal of Laboratory Automation,2012,17(6):443-448.
APA Wang ZB.,Liu LQ.,Wang YC.,Xi N.,Dong ZL.,...&Yuan S.(2012).A Fully Automated System for Measuring Cellular Mechanical Properties.Journal of Laboratory Automation,17(6),443-448.
MLA Wang ZB,et al."A Fully Automated System for Measuring Cellular Mechanical Properties".Journal of Laboratory Automation 17.6(2012):443-448.
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