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A Fully Automated System for Measuring Cellular Mechanical Properties
Wang ZB(王智博); Liu LQ(刘连庆); Wang YC(王越超); Xi N(席宁); Dong ZL(董再励); Li M(李密); Yuan S(袁帅)
作者部门机器人学研究室
关键词Automation Mechanical Properties Of Cells Atomic Force Microscopy (Afm) Local Scan
发表期刊Journal of Laboratory Automation
ISSN2211-0682
2012
卷号17期号:6页码:443-448
收录类别SCI ; EI
EI收录号20132316393556
WOS记录号WOS:000313344200005
产权排序1
摘要

As a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping cellular mechanical properties are mostly carried out manually, resulting in measurements that are time-consuming with low efficiency. In this article, a fully automated system of measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, the cells are recognized using an image-processing method and the relative position of the cell, and the AFM tip is accurately calibrated by the local scan method, meaning that the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. In addition, with the implementation of the automation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. The capability of our system is validated on Raji cells, and the results indicate that the measurement rate of our system is 26 times faster than that of the traditional manual method, providing the technology for high-throughput measurement of cellular mechanical properties.

语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS类目Biochemical Research Methods ; Chemistry, Analytical
关键词[WOS]Atomic-force Microscopy ; Cells ; Afm
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/10330
专题机器人学研究室
通讯作者Liu LQ(刘连庆)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Graduate University of Chinese Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States
推荐引用方式
GB/T 7714
Wang ZB,Liu LQ,Wang YC,et al. A Fully Automated System for Measuring Cellular Mechanical Properties[J]. Journal of Laboratory Automation,2012,17(6):443-448.
APA Wang ZB.,Liu LQ.,Wang YC.,Xi N.,Dong ZL.,...&Yuan S.(2012).A Fully Automated System for Measuring Cellular Mechanical Properties.Journal of Laboratory Automation,17(6),443-448.
MLA Wang ZB,et al."A Fully Automated System for Measuring Cellular Mechanical Properties".Journal of Laboratory Automation 17.6(2012):443-448.
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