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Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy
Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励); Guan, Yifu
Department机器人学研究室
Conference Name4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Conference DateDecember 5-9, 2010
Conference PlaceHong Kong/Macau, China
Author of SourceIEEE Nanotechnology Council; Chinese International NEMS Society; City University of Hong Kong; The Chinese University of Hong Kong; The University of Hong Kong; University of Macau
Source Publication2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
PublisherIEEE Computer Society
Publication PlacePiscataway, USA
2010
Pages125-129
Indexed ByEI
EI Accession number20112013992993
Contribution Rank2
ISBN978-1-61284-153-3
KeywordAtomic Force Microscopy Binding Energy Cluster Analysis Dna Experiments Hydrogen Precision Engineering Sodium Sodium Chloride
Abstract

Atomic force microscope (AFM), as its high resolution and precision, was employed to directly probe intermolecular hydrogen bonds between self-assembled purines and pyrimidines. In the experiment, we directly stretch the DNA double strands that are attached to the planar modified glass surfaces and AFM tip respectively. However, distinguishing from previous literatures we focus on the NaCl density and holding time influencing the rupture force of DNA double helix and monitor the probing process under three different densities of sodium chloride (NaCl): 0mM, 50mM, and 100mM. The result shows complementary DNA strands are easier to bond as the density of NaCl increases. At the same time we test the probing process considering three holding durations: 0S, 10S, and 40S. We find the complementary DNA strands bond forming well under the situation of 40S. Then we separately do experiments with three lengths of strands: 10bp, 14bp, 20bp. Using cluster analysis method we find that the clustering center intervals are about 0.21nN, 0.29nN, and 0.39nN, and we can calculate the hydrogen bond of the single G-C is about 20pN.

Language英语
Document Type会议论文
Identifierhttp://ir.sia.cn/handle/173321/10470
Collection机器人学研究室
Affiliation1.Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
3.Graduate School of the Chinese Academy of Sciences, Shenyang, China
Recommended Citation
GB/T 7714
Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Measurement of interchain binding affinity of nucleic acid duplex using atomic force microscopy[C]//IEEE Nanotechnology Council; Chinese International NEMS Society; City University of Hong Kong; The Chinese University of Hong Kong; The University of Hong Kong; University of Macau. Piscataway, USA:IEEE Computer Society,2010:125-129.
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