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Imaging and measuring the molecular force of lymphoma pathological cells using atomic force microscopy
Li M(李密); Xiao XB(肖秀斌); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Cytology Molecules Monoclonal Antibodies Nanoprobes Oncology Polyethylene Glycols Topography
发表期刊Scanning
ISSN0161-0457
2013
卷号35期号:1页码:40-46
收录类别SCI ; EI
EI收录号20130916049438
WOS记录号WOS:000315270100007
产权排序1
摘要Summary Atomic force microscopy (AFM) provides a new technology to visualize the cellular topography and quantify the molecular interactions at nanometer spatial resolution. In this work, AFM was used to image the cellular topography and measure the molecular force of pathological cells from B-cell lymphoma patients. After the fluorescence staining, cancer cells were recognized by their special morphological features and then the detailed topography was visualized by AFM imaging. The AFM images showed that cancer cells were much rougher than healthy cells. CD20 is a surface marker of B cells and rituximab is a monoclonal antibody against CD20. To measure the CD20-rituximab interaction forces, the polyethylene glycol (PEG) linker was used to link rituximab onto the AFM tip and the verification experiments of the functionalized probe indicated that rituximab molecules were successfully linked onto the AFM tip. The CD20-rituximab interaction forces were measured on about 20 pathological cells and the force measurement results indicated the CD20-rituximab binding forces were mainly in the range of 110-120 pN and 130-140 pN. These results can improve our understanding of the topography and molecular force of lymphoma pathological cells. SCANNING 35:40-46, 2013.
语种英语
WOS标题词Science & Technology ; Technology
WOS类目Instruments & Instrumentation ; Microscopy
关键词[WOS]SINGLE-MOLECULE ; LIVING CELLS ; CANCER ; ANTIBODIES ; AFM
WOS研究方向Instruments & Instrumentation ; Microscopy
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/10630
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Graduate University of Chinese Academy of Sciences, Beijing, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical, Academy of Sciences, Beijing, China
4.Department of Electrical and Computer Engineering, Michigan State University Michigan, East Lansing, United States
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GB/T 7714
Li M,Xiao XB,Liu LQ,et al. Imaging and measuring the molecular force of lymphoma pathological cells using atomic force microscopy[J]. Scanning,2013,35(1):40-46.
APA Li M.,Xiao XB.,Liu LQ.,Xi N.,Wang YC.,...&Zhang WJ.(2013).Imaging and measuring the molecular force of lymphoma pathological cells using atomic force microscopy.Scanning,35(1),40-46.
MLA Li M,et al."Imaging and measuring the molecular force of lymphoma pathological cells using atomic force microscopy".Scanning 35.1(2013):40-46.
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