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题名: Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics
作者: Liang WF(梁文峰) ; Liu B(刘斌) ; Liu LQ(刘连庆) ; Dong ZL(董再励) ; Lee, Gwo-Bin ; Li WR(李文荣) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
会议名称: 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
会议日期: April 7-10, 2013
会议地点: Suzhou, China
会议主办者: Institute of Electrical and Electronics Engineers (IEEE); IEEE Nanotechnology Council (NTC); Peking University; Soochow University; Chinese Academy of Sciences
会议录: 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
会议录出版者: IEEE Computer Society
会议录出版地: Washington, United States
出版日期: 2013
页码: 1253-1256
收录类别: CPCI(ISTP) ; EI
ISBN号: 9781467363525
关键词: Bioassay ; Biochips ; Blood ; Electrodes ; Electrodynamics ; Electrohydrodynamics ; Electromagnetic fields ; Electroosmosis ; Electrophoresis
摘要: This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. © 2013 IEEE.
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内容类型: 会议论文
URI标识: http://ir.sia.cn/handle/173321/12412
Appears in Collections:机器人学研究室_会议论文

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