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Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics
Liang WF(梁文峰); Liu B(刘斌); Liu LQ(刘连庆); Dong ZL(董再励); Lee, Gwo-Bin; Li WJ(李文荣); Zhang WJ(张伟京)
作者部门机器人学研究室
会议名称8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
会议日期April 7-10, 2013
会议地点Suzhou, China
会议主办者Institute of Electrical and Electronics Engineers (IEEE); IEEE Nanotechnology Council (NTC); Peking University; Soochow University; Chinese Academy of Sciences
会议录名称8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
出版者IEEE Computer Society
出版地Washington, United States
2013
页码1253-1256
收录类别EI ; CPCI(ISTP)
EI收录号20133616690605
WOS记录号WOS:000327183000269
产权排序1
ISBN号978-1-4673-6352-5
关键词Bioassay Biochips Blood Electrodes Electrodynamics Electrohydrodynamics Electromagnetic Fields Electroosmosis Electrophoresis
摘要

This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. © 2013 IEEE.

语种英语
引用统计
文献类型会议论文
条目标识符http://ir.sia.cn/handle/173321/12412
专题机器人学研究室
通讯作者Li WJ(李文荣)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of the Chinese Academy of Sciences, Beijing, China
3.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
4.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong
5.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
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Liang WF,Liu B,Liu LQ,et al. Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics[C]//Institute of Electrical and Electronics Engineers (IEEE); IEEE Nanotechnology Council (NTC); Peking University; Soochow University; Chinese Academy of Sciences. Washington, United States:IEEE Computer Society,2013:1253-1256.
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