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题名: Atomic force microscopy imaging of live mammalian cells
作者: Li M(李密) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超) ; Dong ZL(董再励) ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: MECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
刊名: SCIENCE CHINA-LIFE SCIENCES
ISSN号: 1674-7305
出版日期: 2013
卷号: 56, 期号:9, 页码:811-817
收录类别: SCI
产权排序: 1
摘要: Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.
语种: 英语
WOS记录号: WOS:000323669300006
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
关键词[WOS]: MECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/12549
Appears in Collections:机器人学研究室_期刊论文

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李密; 刘连庆; 席宁; 王越超; 董再励; 肖秀斌; 张伟京.Atomic force microscopy imaging of live mammalian cells,SCIENCE CHINA-LIFE SCIENCES,2013,56(9):811-817
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