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Atomic force microscopy imaging of live mammalian cells
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Mechanical-properties Living Cells Motile Cells In-vitro Shape Elasticity Migration Surface Actin
发表期刊SCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
2013
卷号56期号:9页码:811-817
收录类别SCI
WOS记录号WOS:000323669300006
产权排序1
资助机构National Natural Science Foundation of China [61175103]; CAS FEA International Partnership Program for Creative Research Teams
摘要Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biology
关键词[WOS]MECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
WOS研究方向Life Sciences & Biomedicine - Other Topics
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/12549
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Atomic force microscopy imaging of live mammalian cells[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(9):811-817.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2013).Atomic force microscopy imaging of live mammalian cells.SCIENCE CHINA-LIFE SCIENCES,56(9),811-817.
MLA Li M,et al."Atomic force microscopy imaging of live mammalian cells".SCIENCE CHINA-LIFE SCIENCES 56.9(2013):811-817.
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