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Atomic force microscopy imaging of live mammalian cells
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
2013
Volume56Issue:9Pages:811-817
Indexed BySCI
WOS IDWOS:000323669300006
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [61175103]; CAS FEA International Partnership Program for Creative Research Teams
KeywordMechanical-properties Living Cells Motile Cells In-vitro Shape Elasticity Migration Surface Actin
AbstractAtomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiology
WOS KeywordMECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
WOS Research AreaLife Sciences & Biomedicine - Other Topics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/12549
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Zhang WJ(张伟京)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Atomic force microscopy imaging of live mammalian cells[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(9):811-817.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2013).Atomic force microscopy imaging of live mammalian cells.SCIENCE CHINA-LIFE SCIENCES,56(9),811-817.
MLA Li M,et al."Atomic force microscopy imaging of live mammalian cells".SCIENCE CHINA-LIFE SCIENCES 56.9(2013):811-817.
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