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题名:
Atomic force microscopy imaging of live mammalian cells
作者: Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门: 机器人学研究室
通讯作者: 刘连庆 ; 张伟京
关键词: MECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
刊名: SCIENCE CHINA-LIFE SCIENCES
ISSN号: 1674-7305
出版日期: 2013
卷号: 56, 期号:9, 页码:811-817
收录类别: SCI
WOS记录号: WOS:000323669300006
产权排序: 1
摘要: Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.
语种: 英语
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
关键词[WOS]: MECHANICAL-PROPERTIES ; LIVING CELLS ; MOTILE CELLS ; IN-VITRO ; SHAPE ; ELASTICITY ; MIGRATION ; SURFACE ; ACTIN
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/12549
Appears in Collections:机器人学研究室_期刊论文

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作者单位: 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China

Recommended Citation:
Li M,Liu LQ,Xi N,et al. Atomic force microscopy imaging of live mammalian cells[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(9):811-817.
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