SIA OpenIR  > 机器人学研究室
Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition
Li M(李密); Xiao XB(肖秀斌); Liu LQ(刘连庆); Xi N(席宁); Zhang CL(张常麟); Wang YC(王越超); Dong ZL(董再励); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Single-molecule Force Spectroscopy Cd20 Rituximab Ror1 Lymphoma
发表期刊JOURNAL OF MOLECULAR RECOGNITION
ISSN0952-3499
2013
卷号26期号:9页码:432-438
收录类别SCI
WOS记录号WOS:000321441800007
产权排序1
资助机构National Natural Science Foundation of China [61175103]; CAS FEA International Partnership Program for Creative Research Teams
摘要Knowledge of drug-target interaction is critical to our understanding of drug action and can help design better drugs. Due to the lack of adequate single-molecule techniques, the information of individual interactions between ligand-receptors is scarce until the advent of atomic force microscopy (AFM) that can be used to directly measure the individual ligand-receptor forces under near-physiological conditions by linking ligands onto the surface of the AFM tip and then obtaining force curves on cells. Most of the current AFM single-molecule force spectroscopy experiments were performed on cells grown in vitro (cell lines) that are quite different from the human cells in vivo. From the view of clinical practice, investigating the drug-target interactions directly on the patient cancer cells will bring more valuable knowledge that may potentially serve as an important parameter in personalized treatment. Here, we demonstrate the capability of AFM to measure the binding force between target (CD20) and drug (rituximab, an anti-CD20 monoclonal antibody targeted drug) directly on lymphoma patient cancer cells under the assistance of ROR1 fluorescence recognition. ROR1 is a receptor expressed on some B-cell lymphomas but not on normal cells. First, B-cell lymphoma Raji cells (a cell line) were used for ROR1 fluorescence labeling and subsequent measurement of CD20-rituximab binding force. The results showed that Raji cells expressed ROR1, and the labeling of ROR1 did not influence the measurement of CD20-rituximab binding force. Then the established experimental procedures were performed on the pathological samples prepared from the bone marrow of a follicular lymphoma patient. Cancer cells were recognized by ROR1 fluorescence. Under the guidance of fluorescence, with the use of a rituximab-conjugated tip, the cellular topography was visualized by using AFM imaging and the CD20-Rituximab binding force was measured by single-molecule force spectroscopy.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ANTI-CD20 MONOCLONAL-ANTIBODIES ; MOLECULAR RECOGNITION ; LIVING CELLS ; RECEPTOR ; SPECTROSCOPY ; RITUXIMAB ; LYMPHOMA ; THERAPEUTICS ; EXPRESSION ; DRUG
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/12550
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
4.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
推荐引用方式
GB/T 7714
Li M,Xiao XB,Liu LQ,et al. Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition[J]. JOURNAL OF MOLECULAR RECOGNITION,2013,26(9):432-438.
APA Li M.,Xiao XB.,Liu LQ.,Xi N.,Zhang CL.,...&Zhang WJ.(2013).Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition.JOURNAL OF MOLECULAR RECOGNITION,26(9),432-438.
MLA Li M,et al."Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition".JOURNAL OF MOLECULAR RECOGNITION 26.9(2013):432-438.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Atomic force microsc(1051KB)期刊论文作者接受稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li M(李密)]的文章
[Xiao XB(肖秀斌)]的文章
[Liu LQ(刘连庆)]的文章
百度学术
百度学术中相似的文章
[Li M(李密)]的文章
[Xiao XB(肖秀斌)]的文章
[Liu LQ(刘连庆)]的文章
必应学术
必应学术中相似的文章
[Li M(李密)]的文章
[Xiao XB(肖秀斌)]的文章
[Liu LQ(刘连庆)]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。