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题名: Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition
作者: Li M(李密) ; Xiao XB(肖秀斌) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Zhang CL(张常麟) ; Wang YC(王越超) ; Dong ZL(董再励) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: atomic force microscopy ; single-molecule force spectroscopy ; CD20 ; rituximab ; ROR1 ; lymphoma
刊名: JOURNAL OF MOLECULAR RECOGNITION
ISSN号: 0952-3499
出版日期: 2013
卷号: 26, 期号:9, 页码:432-438
收录类别: SCI
产权排序: 1
摘要: Knowledge of drug-target interaction is critical to our understanding of drug action and can help design better drugs. Due to the lack of adequate single-molecule techniques, the information of individual interactions between ligand-receptors is scarce until the advent of atomic force microscopy (AFM) that can be used to directly measure the individual ligand-receptor forces under near-physiological conditions by linking ligands onto the surface of the AFM tip and then obtaining force curves on cells. Most of the current AFM single-molecule force spectroscopy experiments were performed on cells grown in vitro (cell lines) that are quite different from the human cells in vivo. From the view of clinical practice, investigating the drug-target interactions directly on the patient cancer cells will bring more valuable knowledge that may potentially serve as an important parameter in personalized treatment. Here, we demonstrate the capability of AFM to measure the binding force between target (CD20) and drug (rituximab, an anti-CD20 monoclonal antibody targeted drug) directly on lymphoma patient cancer cells under the assistance of ROR1 fluorescence recognition. ROR1 is a receptor expressed on some B-cell lymphomas but not on normal cells. First, B-cell lymphoma Raji cells (a cell line) were used for ROR1 fluorescence labeling and subsequent measurement of CD20-rituximab binding force. The results showed that Raji cells expressed ROR1, and the labeling of ROR1 did not influence the measurement of CD20-rituximab binding force. Then the established experimental procedures were performed on the pathological samples prepared from the bone marrow of a follicular lymphoma patient. Cancer cells were recognized by ROR1 fluorescence. Under the guidance of fluorescence, with the use of a rituximab-conjugated tip, the cellular topography was visualized by using AFM imaging and the CD20-Rituximab binding force was measured by single-molecule force spectroscopy.
语种: 英语
WOS记录号: WOS:000321441800007
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ANTI-CD20 MONOCLONAL-ANTIBODIES ; MOLECULAR RECOGNITION ; LIVING CELLS ; RECEPTOR ; SPECTROSCOPY ; RITUXIMAB ; LYMPHOMA ; THERAPEUTICS ; EXPRESSION ; DRUG
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/12550
Appears in Collections:机器人学研究室_期刊论文

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