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Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Zhang CL(张常麟); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
2013
Volume58Issue:13Pages:1516-1519
Indexed BySCI
WOS IDWOS:000318527200005
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [60904095, 61175103]; CAS
KeywordAtomic Force Microscopy Cd20 Rituximab Lift Scan Force Curve
AbstractAtomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 x 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordRECOGNITION EVENTS ; DNA-MOLECULES ; LIVING CELLS ; AFM ; SPECTROSCOPY ; LOCALIZATION ; NANOSCOPY ; REVEALS
WOS Research AreaScience & Technology - Other Topics
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/12554
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2013,58(13):1516-1519.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Zhang CL.,...&Zhang WJ.(2013).Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy.CHINESE SCIENCE BULLETIN,58(13),1516-1519.
MLA Li M,et al."Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy".CHINESE SCIENCE BULLETIN 58.13(2013):1516-1519.
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