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Progress of AFM single-cell and single-molecule morphology imaging
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic-force Microscopy Living Cells Membrane-proteins Mechanical-properties Spectroscopy Resolution Dynamics Field Nanoscopy Channel
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
2013
卷号58期号:26页码:3177-3182
收录类别SCI
WOS记录号WOS:000323741500002
产权排序1
资助机构National Natural Science Foundation of China [61175103]; CAS FEA International Partnership Program for Creative Research Teams
摘要Atomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallography. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed.
语种英语
WOS标题词Science & Technology
WOS类目Multidisciplinary Sciences
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; LIVING CELLS ; MEMBRANE-PROTEINS ; MECHANICAL-PROPERTIES ; SPECTROSCOPY ; RESOLUTION ; DYNAMICS ; FIELD ; NANOSCOPY ; CHANNEL
WOS研究方向Science & Technology - Other Topics
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/12562
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China 4.Department of LymphomaAffiliated Hospital of Military Medical Academy of SciencesBeijingChina
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Progress of AFM single-cell and single-molecule morphology imaging[J]. CHINESE SCIENCE BULLETIN,2013,58(26):3177-3182.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2013).Progress of AFM single-cell and single-molecule morphology imaging.CHINESE SCIENCE BULLETIN,58(26),3177-3182.
MLA Li M,et al."Progress of AFM single-cell and single-molecule morphology imaging".CHINESE SCIENCE BULLETIN 58.26(2013):3177-3182.
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