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题名: Progress of AFM single-cell and single-molecule morphology imaging
作者: Li M(李密) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超) ; Dong ZL(董再励) ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: ATOMIC-FORCE MICROSCOPY ; LIVING CELLS ; MEMBRANE-PROTEINS ; MECHANICAL-PROPERTIES ; SPECTROSCOPY ; RESOLUTION ; DYNAMICS ; FIELD ; NANOSCOPY ; CHANNEL
刊名: CHINESE SCIENCE BULLETIN
ISSN号: 1001-6538
出版日期: 2013
卷号: 58, 期号:26, 页码:3177-3182
收录类别: SCI
产权排序: 1
摘要: Atomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallography. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed.
语种: 英语
WOS记录号: WOS:000323741500002
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; LIVING CELLS ; MEMBRANE-PROTEINS ; MECHANICAL-PROPERTIES ; SPECTROSCOPY ; RESOLUTION ; DYNAMICS ; FIELD ; NANOSCOPY ; CHANNEL
研究领域[WOS]: Science & Technology - Other Topics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/12562
Appears in Collections:机器人学研究室_期刊论文

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Recommended Citation:
李密; 刘连庆; 席宁; 王越超; 董再励; 肖秀斌; 张伟京.Progress of AFM single-cell and single-molecule morphology imaging,CHINESE SCIENCE BULLETIN,2013,58(26):3177-3182
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