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Progress of AFM single-cell and single-molecule morphology imaging
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
2013
Volume58Issue:26Pages:3177-3182
Indexed BySCI
WOS IDWOS:000323741500002
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [61175103]; CAS FEA International Partnership Program for Creative Research Teams
KeywordAtomic-force Microscopy Living Cells Membrane-proteins Mechanical-properties Spectroscopy Resolution Dynamics Field Nanoscopy Channel
AbstractAtomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallography. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordATOMIC-FORCE MICROSCOPY ; LIVING CELLS ; MEMBRANE-PROTEINS ; MECHANICAL-PROPERTIES ; SPECTROSCOPY ; RESOLUTION ; DYNAMICS ; FIELD ; NANOSCOPY ; CHANNEL
WOS Research AreaScience & Technology - Other Topics
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/12562
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China 4.Department of LymphomaAffiliated Hospital of Military Medical Academy of SciencesBeijingChina
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Progress of AFM single-cell and single-molecule morphology imaging[J]. CHINESE SCIENCE BULLETIN,2013,58(26):3177-3182.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2013).Progress of AFM single-cell and single-molecule morphology imaging.CHINESE SCIENCE BULLETIN,58(26),3177-3182.
MLA Li M,et al."Progress of AFM single-cell and single-molecule morphology imaging".CHINESE SCIENCE BULLETIN 58.26(2013):3177-3182.
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