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题名: Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study
作者: Zhang CL(张常麟) ; Li P(李鹏) ; Liu LQ(刘连庆) ; Wang YC(王越超) ; Gao ZB(高召兵) ; Li GY(李广勇)
作者部门: 机器人学研究室
关键词: Atomic force microscope (AFM) ; mechanosensitive (MS) ion channel ; planar patch clamp
刊名: IEEE/ASME TRANSACTIONS ON MECHATRONICS
ISSN号: 1083-4435
出版日期: 2014
卷号: 19, 期号:4, 页码:1138-1147
收录类别: SCI ; EI
产权排序: 1
摘要: Mechanosensitive ion channels play important roles for sensing and responding to the mechanical stimuli signals in living life. Here we report the development of a mechanostimulated patch-clamp system for simultaneous recording of external stimuli and acquisition of cellular physiological responses. This system integrates a custom-designed planar patch-clamp system with a robot-assisted atomic force microscope (AFM) system. The former, with a microfluidic channel, can realize not only recording electrical signals but also exchanging intracellular solution; while the latter, enhanced by robotic techniques (local scan force feedback, augmented reality vision feedback), can generate force stimuli with controllable patterns and magnitudes under the operator’s real-time monitoring. To verify the performance of the developed system, we first measured the whole-cell current of the voltagegated potassium ion channel Kv1.1 expressed on Human Embryonic Kidney (HEK293) cells and then recorded the mechanosensitive ion channel current in amouse neuroblastoma cell line (Neuro2 A) in the whole-cell configuration during the AFM indenting on the membrane surface; finally, confirmed the ability to exchange intracellular solution by delivering propidium iodide into the captured cell through intracellular solution. The results prove the effectiveness of the system.
WOS记录号: WOS:000335915800004
WOS标题词: Science & Technology ; Technology
类目[WOS]: Automation & Control Systems ; Engineering, Manufacturing ; Engineering, Electrical & Electronic ; Engineering, Mechanical
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; GATED K+ CHANNELS ; MEMBRANE PATCHES ; SENSORY NEURONS ; SILICON CHIP ; ION CHANNELS ; LIVING CELLS ; WHOLE-CELL ; MECHANOTRANSDUCTION ; NANOMANIPULATION
研究领域[WOS]: Automation & Control Systems ; Engineering
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/14057
Appears in Collections:机器人学研究室_期刊论文

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