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Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study
Zhang CL(张常麟); Li P(李鹏); Liu LQ(刘连庆); Wang YC(王越超); Gao ZB(高召兵); Li GY(李广勇)
作者部门机器人学研究室
关键词Atomic Force Microscope (Afm) Mechanosensitive (Ms) Ion Channel Planar Patch Clamp
发表期刊IEEE/ASME TRANSACTIONS ON MECHATRONICS
ISSN1083-4435
2014
卷号19期号:4页码:1138-1147
收录类别SCI ; EI
EI收录号20142117736473
WOS记录号WOS:000335915800004
产权排序1
摘要Mechanosensitive ion channels play important roles for sensing and responding to the mechanical stimuli signals in living life. Here we report the development of a mechanostimulated patch-clamp system for simultaneous recording of external stimuli and acquisition of cellular physiological responses. This system integrates a custom-designed planar patch-clamp system with a robot-assisted atomic force microscope (AFM) system. The former, with a microfluidic channel, can realize not only recording electrical signals but also exchanging intracellular solution; while the latter, enhanced by robotic techniques (local scan force feedback, augmented reality vision feedback), can generate force stimuli with controllable patterns and magnitudes under the operator’s real-time monitoring. To verify the performance of the developed system, we first measured the whole-cell current of the voltagegated potassium ion channel Kv1.1 expressed on Human Embryonic Kidney (HEK293) cells and then recorded the mechanosensitive ion channel current in amouse neuroblastoma cell line (Neuro2 A) in the whole-cell configuration during the AFM indenting on the membrane surface; finally, confirmed the ability to exchange intracellular solution by delivering propidium iodide into the captured cell through intracellular solution. The results prove the effectiveness of the system.
语种英语
WOS标题词Science & Technology ; Technology
WOS类目Automation & Control Systems ; Engineering, Manufacturing ; Engineering, Electrical & Electronic ; Engineering, Mechanical
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; GATED K+ CHANNELS ; MEMBRANE PATCHES ; SENSORY NEURONS ; SILICON CHIP ; ION CHANNELS ; LIVING CELLS ; WHOLE-CELL ; MECHANOTRANSDUCTION ; NANOMANIPULATION
WOS研究方向Automation & Control Systems ; Engineering
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/14057
专题机器人学研究室
通讯作者Zhang CL(张常麟)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
4.Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai 201203, China
5.Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15213, United States
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GB/T 7714
Zhang CL,Li P,Liu LQ,et al. Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study[J]. IEEE/ASME TRANSACTIONS ON MECHATRONICS,2014,19(4):1138-1147.
APA Zhang CL,Li P,Liu LQ,Wang YC,Gao ZB,&Li GY.(2014).Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study.IEEE/ASME TRANSACTIONS ON MECHATRONICS,19(4),1138-1147.
MLA Zhang CL,et al."Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study".IEEE/ASME TRANSACTIONS ON MECHATRONICS 19.4(2014):1138-1147.
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