SIA OpenIR  > 机器人学研究室
Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study
Zhang CL(张常麟); Li P(李鹏); Liu LQ(刘连庆); Wang YC(王越超); Gao ZB(高召兵); Li GY(李广勇)
Department机器人学研究室
Source PublicationIEEE/ASME TRANSACTIONS ON MECHATRONICS
ISSN1083-4435
2014
Volume19Issue:4Pages:1138-1147
Indexed BySCI ; EI
EI Accession number20142117736473
WOS IDWOS:000335915800004
Contribution Rank1
KeywordAtomic Force Microscope (Afm) Mechanosensitive (Ms) Ion Channel Planar Patch Clamp
AbstractMechanosensitive ion channels play important roles for sensing and responding to the mechanical stimuli signals in living life. Here we report the development of a mechanostimulated patch-clamp system for simultaneous recording of external stimuli and acquisition of cellular physiological responses. This system integrates a custom-designed planar patch-clamp system with a robot-assisted atomic force microscope (AFM) system. The former, with a microfluidic channel, can realize not only recording electrical signals but also exchanging intracellular solution; while the latter, enhanced by robotic techniques (local scan force feedback, augmented reality vision feedback), can generate force stimuli with controllable patterns and magnitudes under the operator’s real-time monitoring. To verify the performance of the developed system, we first measured the whole-cell current of the voltagegated potassium ion channel Kv1.1 expressed on Human Embryonic Kidney (HEK293) cells and then recorded the mechanosensitive ion channel current in amouse neuroblastoma cell line (Neuro2 A) in the whole-cell configuration during the AFM indenting on the membrane surface; finally, confirmed the ability to exchange intracellular solution by delivering propidium iodide into the captured cell through intracellular solution. The results prove the effectiveness of the system.
Language英语
WOS HeadingsScience & Technology ; Technology
WOS SubjectAutomation & Control Systems ; Engineering, Manufacturing ; Engineering, Electrical & Electronic ; Engineering, Mechanical
WOS KeywordATOMIC-FORCE MICROSCOPY ; GATED K+ CHANNELS ; MEMBRANE PATCHES ; SENSORY NEURONS ; SILICON CHIP ; ION CHANNELS ; LIVING CELLS ; WHOLE-CELL ; MECHANOTRANSDUCTION ; NANOMANIPULATION
WOS Research AreaAutomation & Control Systems ; Engineering
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/14057
Collection机器人学研究室
Corresponding AuthorZhang CL(张常麟)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
4.Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai 201203, China
5.Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15213, United States
Recommended Citation
GB/T 7714
Zhang CL,Li P,Liu LQ,et al. Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study[J]. IEEE/ASME TRANSACTIONS ON MECHATRONICS,2014,19(4):1138-1147.
APA Zhang CL,Li P,Liu LQ,Wang YC,Gao ZB,&Li GY.(2014).Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study.IEEE/ASME TRANSACTIONS ON MECHATRONICS,19(4),1138-1147.
MLA Zhang CL,et al."Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study".IEEE/ASME TRANSACTIONS ON MECHATRONICS 19.4(2014):1138-1147.
Files in This Item: Download All
File Name/Size DocType Version Access License
Development of Mecha(865KB)期刊论文作者接受稿开放获取ODC PDDLView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang CL(张常麟)]'s Articles
[Li P(李鹏)]'s Articles
[Liu LQ(刘连庆)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang CL(张常麟)]'s Articles
[Li P(李鹏)]'s Articles
[Liu LQ(刘连庆)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang CL(张常麟)]'s Articles
[Li P(李鹏)]'s Articles
[Liu LQ(刘连庆)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.