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Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
2013
Volume438Issue:4Pages:709-714
Indexed BySCI
WOS IDWOS:000324454800023
Contribution Rank1
Funding OrganizationThis work was supported by the National Natural Science Foundation of China (61175103) and CAS FEA International Partnership Program for Creative Research Teams.
KeywordAtomic Force Microscopy Immunotherapy Antibody Macrophage Fc Gamma Receptor
AbstractFc gamma receptors (Fc gamma R), widely expressed on effector cells (e.g., NK cells, macrophages), play an important role in clinical cancer immunotherapy. The binding of Fc gamma Rs to the Fc portions of antibodies that are attached to the target cells can activate the antibody-dependent cell-mediated cytotoxicity (ADCC) killing mechanism which leads to the lysis of target cells. In this work, we used atomic force microscopy (AFM) to observe the cellular ultra-structures and measure the biophysical properties (affinity and distribution) of Fc gamma Rs on single macrophages in aqueous environments. AFM imaging was used to obtain the topographies of macrophages, revealing the nanoscale cellular fine structures. For molecular interaction recognition, antibody molecules were attached onto AFM tips via a heterobifunctional polyethylene glycol (PEG) crosslinker. With AFM single-molecule force spectroscopy, the binding affinities of Fc gamma Rs were quantitatively measured on single macrophages. Adhesion force mapping method was used to localize the Fc gamma Rs, revealing the nanoscale distribution of Fc gamma Rs on local areas of macrophages. The experimental results can improve our understanding of Fc gamma Rs on macrophages; the established approach will facilitate further research on physiological activities involved in antibody-based immunotherapy.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS KeywordANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; RITUXIMAB RESISTANCE ; SPECTROSCOPY ; THERAPY ; CANCER ; RECOGNITION ; INFLAMMATION ; MECHANISM ; LYMPHOMA
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/14064
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Zhang WJ(张伟京)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,438(4):709-714.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2013).Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,438(4),709-714.
MLA Li M,et al."Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 438.4(2013):709-714.
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