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Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Immunotherapy Antibody Macrophage Fc Gamma Receptor
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
2013
卷号438期号:4页码:709-714
收录类别SCI
WOS记录号WOS:000324454800023
产权排序1
资助机构This work was supported by the National Natural Science Foundation of China (61175103) and CAS FEA International Partnership Program for Creative Research Teams.
摘要Fc gamma receptors (Fc gamma R), widely expressed on effector cells (e.g., NK cells, macrophages), play an important role in clinical cancer immunotherapy. The binding of Fc gamma Rs to the Fc portions of antibodies that are attached to the target cells can activate the antibody-dependent cell-mediated cytotoxicity (ADCC) killing mechanism which leads to the lysis of target cells. In this work, we used atomic force microscopy (AFM) to observe the cellular ultra-structures and measure the biophysical properties (affinity and distribution) of Fc gamma Rs on single macrophages in aqueous environments. AFM imaging was used to obtain the topographies of macrophages, revealing the nanoscale cellular fine structures. For molecular interaction recognition, antibody molecules were attached onto AFM tips via a heterobifunctional polyethylene glycol (PEG) crosslinker. With AFM single-molecule force spectroscopy, the binding affinities of Fc gamma Rs were quantitatively measured on single macrophages. Adhesion force mapping method was used to localize the Fc gamma Rs, revealing the nanoscale distribution of Fc gamma Rs on local areas of macrophages. The experimental results can improve our understanding of Fc gamma Rs on macrophages; the established approach will facilitate further research on physiological activities involved in antibody-based immunotherapy.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; RITUXIMAB RESISTANCE ; SPECTROSCOPY ; THERAPY ; CANCER ; RECOGNITION ; INFLAMMATION ; MECHANISM ; LYMPHOMA
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/14064
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
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GB/T 7714
Li M,Liu LQ,Xi N,et al. Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,438(4):709-714.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2013).Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,438(4),709-714.
MLA Li M,et al."Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 438.4(2013):709-714.
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