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题名: Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy
作者: Li M(李密) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超) ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: Atomic force microscopy ; Immunotherapy ; Antibody ; Macrophage ; Fc gamma receptor
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN号: 0006-291X
出版日期: 2013
卷号: 438, 期号:4, 页码:709-714
收录类别: SCI
产权排序: 1
摘要: Fc gamma receptors (Fc gamma R), widely expressed on effector cells (e.g., NK cells, macrophages), play an important role in clinical cancer immunotherapy. The binding of Fc gamma Rs to the Fc portions of antibodies that are attached to the target cells can activate the antibody-dependent cell-mediated cytotoxicity (ADCC) killing mechanism which leads to the lysis of target cells. In this work, we used atomic force microscopy (AFM) to observe the cellular ultra-structures and measure the biophysical properties (affinity and distribution) of Fc gamma Rs on single macrophages in aqueous environments. AFM imaging was used to obtain the topographies of macrophages, revealing the nanoscale cellular fine structures. For molecular interaction recognition, antibody molecules were attached onto AFM tips via a heterobifunctional polyethylene glycol (PEG) crosslinker. With AFM single-molecule force spectroscopy, the binding affinities of Fc gamma Rs were quantitatively measured on single macrophages. Adhesion force mapping method was used to localize the Fc gamma Rs, revealing the nanoscale distribution of Fc gamma Rs on local areas of macrophages. The experimental results can improve our understanding of Fc gamma Rs on macrophages; the established approach will facilitate further research on physiological activities involved in antibody-based immunotherapy.
语种: 英语
WOS记录号: WOS:000324454800023
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; RITUXIMAB RESISTANCE ; SPECTROSCOPY ; THERAPY ; CANCER ; RECOGNITION ; INFLAMMATION ; MECHANISM ; LYMPHOMA
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/14064
Appears in Collections:机器人学研究室_期刊论文

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