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题名:
Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients
作者: Li M(李密); Xiao XB(肖秀斌); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Zhang WJ(张伟京)
作者部门: 机器人学研究室
通讯作者: 刘连庆 ; 席宁 ; 张伟京
关键词: Atomic force microscopy ; Non-Hodgkin's lymphoma ; Cancer cell ; CD20 ; Rituximab ; ROR1
刊名: EXPERIMENTAL CELL RESEARCH
ISSN号: 0014-4827
出版日期: 2013
卷号: 319, 期号:18, 页码:2812-2821
收录类别: SCI
WOS记录号: WOS:000326432700006
产权排序: 1
摘要: CD20, a membrane protein highly expressed on most B-cell lymphomas, is an effective target demonstrated in clinical practice for treating B-cell non-Hodgkin's lymphoma (NHL). Rituximab is a monoclonal antibody against CD20. In this work, we applied atomic force microscopy (AFM) to map the nanoscale distribution of CD20 molecules on the surface of cancer cells from clinical B-cell NHL patients under the assistance of ROR1 fluorescence recognition (ROR1 is a specific cell surface marker exclusively expressed on cancer cells). First, the ROR1 fluorescence labeling experiments showed that ROR1 was expressed on cancer cells from B-cell lymphoma patients, but not on normal cells from healthy volunteers. Next, under the guidance of ROR1 fluorescence, the rituximab-conjugated AFM tips were moved to cancer cells to image the cellular morphologies and detect the CD20-rituximab interactions on the cell surfaces. The distribution maps of CD20 on cancer cells were constructed by obtaining arrays of (16 x 16) force curves in local areas (500 x 500 nm(2)) on the cell surfaces. The experimental results provide a new approach to directly investigate the nanoscale distribution of target protein on single clinical cancer cells.
语种: 英语
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Oncology ; Cell Biology
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; ANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; SINGLE-MOLECULE ; CD20 EXPRESSION ; LIPID RAFTS ; ROR1 ; THERAPY ; SURFACE ; SPECTROSCOPY
研究领域[WOS]: Oncology ; Cell Biology
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/14067
Appears in Collections:机器人学研究室_期刊论文

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作者单位: 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China
4.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China

Recommended Citation:
Li M,Xiao XB,Liu LQ,et al. Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients[J]. EXPERIMENTAL CELL RESEARCH,2013,319(18):2812-2821.
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