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Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients
Li M(李密); Xiao XB(肖秀斌); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Non-hodgkin's Lymphoma Cancer Cell Cd20 Rituximab Ror1
发表期刊EXPERIMENTAL CELL RESEARCH
ISSN0014-4827
2013
卷号319期号:18页码:2812-2821
收录类别SCI
WOS记录号WOS:000326432700006
产权排序1
资助机构This work was supported by the National Natural Science Foundation of China (61175103) and CAS FEA International Partnership Program for Creative Research Teams.
摘要CD20, a membrane protein highly expressed on most B-cell lymphomas, is an effective target demonstrated in clinical practice for treating B-cell non-Hodgkin's lymphoma (NHL). Rituximab is a monoclonal antibody against CD20. In this work, we applied atomic force microscopy (AFM) to map the nanoscale distribution of CD20 molecules on the surface of cancer cells from clinical B-cell NHL patients under the assistance of ROR1 fluorescence recognition (ROR1 is a specific cell surface marker exclusively expressed on cancer cells). First, the ROR1 fluorescence labeling experiments showed that ROR1 was expressed on cancer cells from B-cell lymphoma patients, but not on normal cells from healthy volunteers. Next, under the guidance of ROR1 fluorescence, the rituximab-conjugated AFM tips were moved to cancer cells to image the cellular morphologies and detect the CD20-rituximab interactions on the cell surfaces. The distribution maps of CD20 on cancer cells were constructed by obtaining arrays of (16 x 16) force curves in local areas (500 x 500 nm(2)) on the cell surfaces. The experimental results provide a new approach to directly investigate the nanoscale distribution of target protein on single clinical cancer cells.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Oncology ; Cell Biology
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; ANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; SINGLE-MOLECULE ; CD20 EXPRESSION ; LIPID RAFTS ; ROR1 ; THERAPY ; SURFACE ; SPECTROSCOPY
WOS研究方向Oncology ; Cell Biology
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/14067
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China
4.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
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GB/T 7714
Li M,Xiao XB,Liu LQ,et al. Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients[J]. EXPERIMENTAL CELL RESEARCH,2013,319(18):2812-2821.
APA Li M.,Xiao XB.,Liu LQ.,Xi N.,Wang YC.,...&Zhang WJ.(2013).Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients.EXPERIMENTAL CELL RESEARCH,319(18),2812-2821.
MLA Li M,et al."Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients".EXPERIMENTAL CELL RESEARCH 319.18(2013):2812-2821.
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