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Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection
Wang, Chih-Hung; Lee, You-Hsun; Kuo, Hsin-Tzu; Liang WF(梁文峰); Li WJ(李文荣); Lee, Gwo-Bin
作者部门机器人学研究室
发表期刊LAB ON A CHIP
ISSN1473-0197
2014
卷号14期号:3页码:592-601
收录类别SCI
WOS记录号WOS:000328911400018
产权排序3
资助机构National Science Council in Taiwan [NSC102-2218-E-007-001]; Towards a World-Class University
摘要Gene transfection is an important technology for various biological applications. The exogenous DNA is commonly delivered into cells by using a strong electrical field to form transient pores in cellular membranes. However, the high voltage required in this electroporation process may cause cell damage. In this study, a dielectrophoretically-assisted electroporation was developed by using light-activated virtual microelectrodes in a new microfluidic platform. The DNA electrotransfection used a low applied voltage and an alternating current to enable electroporation and transfection. Single or triple fluorescencecarrying plasmids were effectively transfected into various types of mammalian cells, and the fluorescent proteins were successfully expressed in live transfected cells. Moreover, the multi-triangle optical pattern that was projected onto a photoconductive layer to generate localized non-uniform virtual electric fields was found to have high transfection efficiency. The developed dielectrophoretically-assisted electroporation platform may provide a simpler system for gene transfection and could be widely applied in many biotechnological fields.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS类目Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
关键词[WOS]CELL-LYSIS DEVICE ; GENE DELIVERY ; ELECTRIC-FIELD ; TISSUE ; SEPARATION ; SYSTEM ; MICRO
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/14716
专题机器人学研究室
通讯作者Lee, Gwo-Bin
作者单位1.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
2.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong
3.State Key Lab of Robotics, Shenyang Institute of Automation, Chinese Academy of Science, Shenyang, China
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GB/T 7714
Wang, Chih-Hung,Lee, You-Hsun,Kuo, Hsin-Tzu,et al. Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection[J]. LAB ON A CHIP,2014,14(3):592-601.
APA Wang, Chih-Hung,Lee, You-Hsun,Kuo, Hsin-Tzu,Liang WF,Li WJ,&Lee, Gwo-Bin.(2014).Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection.LAB ON A CHIP,14(3),592-601.
MLA Wang, Chih-Hung,et al."Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection".LAB ON A CHIP 14.3(2014):592-601.
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