SIA OpenIR  > 机器人学研究室
Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Antigen-antibody Reactions Atomic Force Microscopy Biomechanics Fluorescence Fluorescence Microscopy Mechanical Properties Nanotechnology Optical Data Storage Optical Microscopy
发表期刊Langmuir
ISSN0743-7463
2014
卷号30期号:6页码:1609-1621
收录类别SCI ; EI
EI收录号20140917390420
WOS记录号WOS:000331774400017
产权排序1
摘要Fc receptor-mediated macrophage phagocytosis against cancer cells is an important mechanism in the immune therapy of cancers. Traditional research about macrophage phagocytosis was based on optical microscopy, which cannot reveal detailed information because of the 200-nm-resolution limit. Quantitatively investigating the macrophage phagocytosis at micro- and nanoscale levels is still scarce. The advent of atomic force microscopy (AFM) offers an excellent analytical instrument for quantitatively investigating the biological processes at single-cell and single-molecule levels under native conditions. In this work, we combined AFM and fluorescence microscopy to visualize and quantify the detailed changes in cell morphology and mechanical properties during the process of Fc receptor-mediated macrophage phagocytosis against cancer cells. Lymphoma cells were discernible by fluorescence staining. Then, the dynamic process of phagocytosis was observed by time-lapse optical microscopy. Next, AFM was applied to investigate the detailed cellular behaviors during macrophage phagocytosis under the guidance of fluorescence recognition. AFM imaging revealed the distinct features in cellular ultramicrostructures for the different steps of macrophage phagocytosis. AFM cell mechanical property measurements indicated that the binding of cancer cells to macrophages could make macrophages become stiffer. The experimental results provide novel insights in understanding the Fc-receptor-mediated macrophage phagocytosis.
语种英语
WOS标题词Science & Technology ; Physical Sciences ; Technology
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; ANTIBODY-BASED IMMUNOTHERAPY ; MONOCLONAL-ANTIBODIES ; LIVING CELLS ; MOTILE CELLS ; AFM ; CYTOSKELETON ; INFECTION ; TARGET ; HETEROGENEITY
WOS研究方向Chemistry ; Materials Science
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/14728
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, Hong Kong
4.Department of Lymphoma, Affiliated Hospital, Military Medical Academy of Sciences, Beijing 100071, China
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells[J]. Langmuir,2014,30(6):1609-1621.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2014).Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells.Langmuir,30(6),1609-1621.
MLA Li M,et al."Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells".Langmuir 30.6(2014):1609-1621.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Nanoscale imaging an(2472KB)期刊论文出版稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
百度学术
百度学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
必应学术
必应学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。