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基于光诱导电液动力学的微纳操控方法与应用研究
其他题名Method and Application Research of Optically-Induced Electrokinetics for Micro/Nano Manipulation
梁文峰1,2
导师李文荣 ; 董再励
分类号TB383
关键词光诱导电液动力学 光诱导介电泳 微纳材料分离与组装 细胞筛选 细胞物性检测
索取号TB383/L49/2014
页数112页
学位专业机械电子工程
学位名称博士
2014-05-17
学位授予单位中国科学院沈阳自动化研究所
学位授予地点沈阳
作者部门机器人学研究室
摘要微纳米科技的发展,极大的促进了人类科技文明的进步。微纳米科技发展,很大程度上取决于微纳尺度操控技术的进步,微纳尺度的操控水平已成为微纳科技发展的重要标志。与宏观尺度不同,微纳尺度的操控面临更大的难度和更多的挑战。因此,研究可实现微纳尺度操控的新的理论方法,成为近年来的研究热点。本论文针对微纳米材料与生物领域面临的需求与挑战,围绕光诱导电液动力学所涉及的科学问题与实现技术,重点开展了基于光诱导电液动力学的微纳操控方法与应用研究。本文的主要研究工作如下:(1) 光诱导电液动力学理论方法研究。研究构建了光电子镊实验系统,建立了光电子镊芯片等效电路模型;在此基础上,运用有限元仿真方法,研究分析了光诱导空间非均匀电场、作用于微纳物体的光诱导介电泳力、交流电渗流、交流电热流的空间分布态,给出了光诱导电液动力学方法的各参量作用与操控范围。(2) 光诱导介电泳力控制理论研究。基于理论分析和实验,重点研究了入射光谱与外加交流电压波形对作用于微纳物体的光诱导介电泳力的影响,建立了光谱与外加交流电压波形与光诱导介电泳力的输入输出关系;理论分析与实验研究表明:(a) 光诱导介电泳力与入射光谱之间满足玻尔兹曼分布;(b) 光诱导介电泳力的大小与外加交流电压波形相关。因此,通过调整光谱与外加电压参数,可建立光诱导电液动力学操控机制。(3) 基于光诱导电液动力学的微纳米材料操控方法研究。在上述研究基础上,以聚苯乙烯微球为对象,系统开展了光诱导介电泳力操控条件研究,建立了尺寸、溶液电导率与交越频率之间关系,给出了可实现多种尺寸聚苯乙烯微球操控与分离的尺寸与溶液电导率条件;完成了基于改变溶液电导率的1 μm与10 μm聚苯乙烯微球的有效分离实验;完成了基于不同方向及不同强度光诱导介电泳力的500 nm、1 μm与10 μm三种尺寸聚苯乙烯微球的同时操控与分离实验。研究了纳米尺度物体的光诱导电液动力学频谱特性;实现了基于交流电渗流作用的50 nm碳纳米颗粒操控实验;完成了基于光诱导介电泳力与交流电渗流共同作用的100 nm金纳米颗粒的图形化装配实验;这为纳米尺度物体操控提供了有意义的理论依据与实现方法。(4) 基于光诱导电液动力学的细胞筛选研究。基于双层核壳结构的极化模型,研究了Raji细胞与血红细胞的光诱导介电泳力作用,分析了这两类细胞的尺寸、形态、电特性因素不同导致的介电泳力差异性,给出了可实现两类细胞分离的确定性条件;并通过五种不同浓度比的Raji细胞与血红细胞分离实验,验证了研究结果的合理性与正确性。(5) 基于光诱导介电泳力的生物细胞物性测量研究。开展了光诱导介电泳力作用下的Raji细胞平移特性实验研究,并依据细胞的极化机制,得出了Raji细胞膜电容检测方法;开展了Raji细胞在光诱导电场中的自旋转行为实验研究,结合计算机视觉检测方法,得出了Raji细胞的自旋转频谱特性;基于有限元仿真研究,证明了光诱导电场的非均匀性与非旋转性,推论出非均匀电场可引起细胞的自旋转行为。本论文的研究工作丰富了光诱导电液动力学理论,证明了光诱导电液动力学技术在微纳米材料操控、分离、装配方面的优势。同时,本文的研究工作也证明了光诱导电液动力学技术是一种能实现细胞的无损、免标记与快速筛选以及研究细胞物性特征的技术。
其他摘要The development of the micro/nano science and technology has promoted the evolvement of human civilization tremendously. The advancement of the micro/nano science and technology highly depends on the progress of the micro/nano manipulation techniques, and the micro/nano-scaled manipulation level is the critical sign of the micro/nano science and technology. The micro/nano manipulation is even more difficulty and challenge than the macro scale. Hence, the research of the novel theory method for the micro/nano-scaled manipulation is currently a trending research topic.This dissertation, aimed at the demand and the challenge of the micro/nano material and biomedical fields and related to the scientific issues and implementation techniques of the optically-induced electrokinetics (OEK) technique, mainly focuses on the method and application research of micro/nano manipulation by the OEK technique. The pivotal research work in this dissertation is summarized as follows: (1) The theoretical study of OEK was presented. The experimental system of the optoelectronic tweezers (OET) was constructed, and the equivalent electric module of the OET chip was also established. After then, the spatial distributions of the optically-induced non-uniform electric field, optically-induced dielectrophoresis (ODEP) exerted on the micro/nano entities, the AC electroosmotic (ACEO), and the AC electrothermal were analyzed by using the finite element method (FEM) simulation, respectively. The various parameters and the manipulation ranges of the OEK technique were elucidated.(2) The control scheme of the ODEP force was discussed. Based on both of the theory and the experiment, the ODEP force dependencies on the optical spectrum and the waveform of the AC bias potential were studied, respectively, and the input-output relationship of the ODEP force was described, revealing two points: (a) The relationship between the optical spectrum and the ODEP force met the Boltzmann distribution; (b) The magnitude of the ODEP force was related with the waveform of the AC bias potential. Accordingly, the manipulation mechanism of the OEK was established by adjusting the optical spectrum and/or the waveform of the AC bias potential.(3) The research of the OEK technique at the micro/nano scale manipulation was described. Based on the aforementioned research, the manipulation of polystyrene beads by utilizing the ODEP force was systematically studied. By exploring the relationship of different sizes of the polystyrene beads, the crossover frequencies, and the liquid conductivities, the manipulation and separation parameters for various sizes of polystyrene beads were given. By adjusting the liquid conductivity, 1 μm and 10 μm polystyrene beads were successfully separated, and then the simultaneous separation of 500 nm, 1 μm, and 10 μm polystyrene beads was also validated by applying different magnitudes and directions of the ODEP forces. The FEM simulation of the frequency spectrum property of the OEK for nano-scaled manipulation was discussed. The effective manipulation of carbon nanoparticles with a diameter of 50 nm was performed based on the ACEO approach, and the graphic assembly of gold nanoparticls with a diameter of 100 nm was achieved with the assist of the ODEP and the ACEO schemes. This study provided a meaningful procedure for rapid manipulation and assembly of nano entities.(4) The cell separation by using OEK was investigated. By applying the double layer structure of the shell-core polarization model, the different ODEP forces for Raji cells and red blood cells (RBCs) were studied due to the different sizes, geometrical configurations, and inherent electrical properties of them. The separation parameters that the ODEP force could be employed to effectively separate Raji cells from RBCs were obtained. The separation of Raji cells from RBCs with five different concentration ratios was experimentally performed, validating that the theory on the cell separation by the ODEP force was reasonable and correct. (5) The measurement of the cellular physical property by using ODEP was studied. Based on the polarization mechanism of cells, the membrane capacitance of Raji cells was extracted by analyzing the shifted translational motion of the cells due to the ODEP force. The self-rotational behavior of Raji cells in the optically-induced electric field was explored and investigated, and the self-rotational spectrum of Raji cells was also characterized with the aid of the computer vision. The FEM simulation results of this electric field confirmed that the self-rotation behavior of cells took place in a non-uniform and non-rotational electric field. Therefore, this unique phenomenon is most likely to be caused by the electrical field non-uniformity.The research work proposed in this study enriches the theory of the OEK. Furthermore, the research work presented in this dissertation verifies the advantages of the OEK technique for the manipulation, separation, and assembly of micro/nano materials and also demonstrates that the OEK technique is a non-destructive, label-free, and rapid approach for cell separation and detection of the cellular physical property.
语种中文
产权排序1
文献类型学位论文
条目标识符http://ir.sia.cn/handle/173321/14834
专题机器人学研究室
作者单位1.中国科学院沈阳自动化研究所
2.中国科学院大学
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梁文峰. 基于光诱导电液动力学的微纳操控方法与应用研究[D]. 沈阳. 中国科学院沈阳自动化研究所,2014.
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