The invention discloses a heart chamber simulator driven by a hydraulic or air-pressure artificial muscle. The heart chamber simulator can really reflect cardiac systolic and diastolic beating process pressure and chamber deformation. The simulator comprises an outer sheath layer, a myocardium layer, cardiac valves and a closed hollow cavity formed between the outer sheath layer and the myocardium layer. The wall surface of the outer sheath layer is provided with a through hole used for input or output of liquid (gas) in the hollow cavity. Contraction and relaxation of a heart model can be achieved by changing the pressure in the hollow cavity. The heart chamber simulator can overcome the defect that a contraction and relaxation pressure error is caused by a pump which is used in an external circulation pipeline and solve the problem that a difference between the heart chamber deformation caused by external mechanical loading and the actual myocardial contraction and relaxation process deformation is large, and really reflects the heart chamber beating process deformation and correct circulating pressure relationships.