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扫描离子电导显微镜的研制与实现
Alternative TitleDevelopment and implementation of scanning ion conductance microscope
李鹏; 张常麟; 王文学; 刘连庆
Department机器人学研究室
Source Publication红外与激光工程
ISSN1007-2276
2014
Volume43Issue:6Pages:1894-1898
Indexed ByEI ; CSCD
EI Accession number20142917960085
CSCD IDCSCD:5177301
Contribution Rank1
Funding Organization国家自然科学基金(61175103); 中国科学院科研装备研制项目(YZ201245); 中国科学院与国家外国专家局创新团队国际合作伙伴计划
Keyword扫描离子电导显微镜 活细胞无损成像 跳跃模式
Abstract在微纳米尺度上对活细胞高分辨率成像对生命科学研究具有重要的意义,其将有助于再现正在发生的生命过程、检测细胞对外界刺激做出的响应,甚至观测某些蛋白簇在细胞膜表面的运动。然而直到今天,仍然没有很好的实现上述目标。扫描离子电导显微镜(SICM)由于其真正的非接触、高分辨、无损独特成像方式,规避了扫描过程中探针与样品表面发生力的接触,得到越来越多的关注和广泛的应用。从系统的角度阐述自制SICM系统的设计、硬件集成及跳跃模式扫描算法的实现,并通过对聚二甲基硅氧烷(PDMS)栅格成像以及与原子力显微镜(AFM)成像结果的对比,验证了系统功能的正确性和有效性;最后开展了生理环境下活体细胞的原位扫描成像实验,...
Other AbstractHigh-resolution imaging of living cell at the micro-/nano-scale is important for life science research. It may help to observe biological activities of cells, and to detect cell responses to external stimuli and even movements of some protein molecules in cell membranes. However, there have not been effective methods to realize such objectives yet. Scanning ion conductance microscope (SICM) has been widely applied in many fields and is receiving increasing attention due to its non-contact, force-free, and high-resolution imaging features. Herein, a design of SICM, including hardware integration and scanning algorithms, was introduced from the point of view of system firstly; then the feasibility and effectiveness of the system was evaluated through comparison of PDMS gratings measurements by SICM and AFM; finally, in situ experiments of living-cell imaging in physiological environment had been carried out, and the topography of living neuro-2A cell had been successfully obtained. The well-established scanning ion conductance microscope will provide an effective tool for investigating functional mechanism and microstructure on the surface of living biological samples.
Language中文
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/15251
Collection机器人学研究室
Corresponding Author刘连庆
Affiliation1.中国科学院沈阳自动化研究所机器人学国家重点实验室
2.中国科学院大学
Recommended Citation
GB/T 7714
李鹏,张常麟,王文学,等. 扫描离子电导显微镜的研制与实现[J]. 红外与激光工程,2014,43(6):1894-1898.
APA 李鹏,张常麟,王文学,&刘连庆.(2014).扫描离子电导显微镜的研制与实现.红外与激光工程,43(6),1894-1898.
MLA 李鹏,et al."扫描离子电导显微镜的研制与实现".红外与激光工程 43.6(2014):1894-1898.
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