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Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超)
Department机器人学研究室
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
2014
Volume59Issue:31Pages:4020-4029
Indexed BySCI
WOS IDWOS:000342421100011
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [61175103, 61327014]; CAS FEA International Partnership Program for Creative Research Teams
KeywordAtomic Force Microscopy Cellular Mechanical Properties Indentation Force Curve Elastic Modulus
AbstractThe advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors’ own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordCANCER-CELLS ; TUMOR PROGRESSION ; AFM-CANTILEVERS ; ELASTIC-MODULUS ; STIFFNESS ; DYNAMICS ; CYTOSKELETON ; SURFACE ; SHAPE ; DEFORMABILITY
WOS Research AreaScience & Technology - Other Topics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/15264
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical EngineeringCity University of Hong Kong, Hong Kong, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2014,59(31):4020-4029.
APA Li M,Liu LQ,Xi N,&Wang YC.(2014).Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy.CHINESE SCIENCE BULLETIN,59(31),4020-4029.
MLA Li M,et al."Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy".CHINESE SCIENCE BULLETIN 59.31(2014):4020-4029.
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