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题名: AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells
作者: Li M(李密) ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超)
作者部门: 机器人学研究室
关键词: Atomic force microscopy ; Antibody-dependent cellular cytotoxicity ; Lymphoma ; Natural killer cell ; CD20 ; Fc receptor
刊名: CELLULAR IMMUNOLOGY
ISSN号: 0008-8749
出版日期: 2014
卷号: 290, 期号:2, 页码:233-244
收录类别: SCI
产权排序: 1
摘要: Rituximab is a monoclonal antibody drug approved for the treatment of patients with lymphomas. Rituximab's main killing mechanism is antibody-dependent cellular cytotoxicity (ADCC). During ADCC, rituximab's fragment antigen binding (Fab) region binds to the CD20 antigen on the tumor cell and its fragment crystallizable (Fc) region binds to the Fc receptor (FcR) on the natural killer (NK) cells. In this study, two types of molecular interactions (CD20-rituximab, FcR-rituximab) involved in ADCC were measured simultaneously on cells prepared from biopsy specimens of lymphoma patients by utilizing atomic force microscopy (AFM) with functionalized tips carrying rituximab. NK cells were detected by specific NKp46 fluorescent labeling and tumor cells were detected by specific ROR1 fluorescent labeling. Based on the fluorescence recognition, the binding affinity and distribution of FcRs on NK cells, and CD20 on tumor cells, were quantitatively measured and mapped. The binding affinity and distribution of FcRs (on NK cells) and CD20 (on tumor cells) were associated with rituximab clinical efficacy. The experimental results provide a new approach to simultaneously quantify the multiple types of molecular interactions involved in rituximab ADCC mechanism on patient biopsy cells, which is of potential clinical significance to predict rituximab efficacy for personalized medicine. (C) 2014 Published by Elsevier Inc.
语种: 英语
WOS记录号: WOS:000342955000009
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Cell Biology ; Immunology
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; NATURAL-KILLER-CELLS ; FC-GAMMA RECEPTORS ; ANTI-CD20 MONOCLONAL-ANTIBODIES ; SINGLE-MOLECULE ; LIVING CELLS ; CANCER-CELLS ; SURFACE EXPRESSION ; RECOGNITION EVENTS ; CLINICAL-EFFICACY
研究领域[WOS]: Cell Biology ; Immunology
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/15268
Appears in Collections:机器人学研究室_期刊论文

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李密; 肖秀斌; 张伟京; 刘连庆; 席宁; 王越超.AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells,CELLULAR IMMUNOLOGY,2014,290(2):233-244
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文件名: AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells.pdf
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