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AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells
Li M(李密); Xiao XB(肖秀斌); Zhang WJ(张伟京); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超)
作者部门机器人学研究室
关键词Atomic Force Microscopy Antibody-dependent Cellular Cytotoxicity Lymphoma Natural Killer Cell Cd20 Fc Receptor
发表期刊CELLULAR IMMUNOLOGY
ISSN0008-8749
2014
卷号290期号:2页码:233-244
收录类别SCI
WOS记录号WOS:000342955000009
产权排序1
资助机构National Natural Science Foundation of China [61175103, 61327014]; CAS FEA International Partnership Program for Creative Research Teams
摘要Rituximab is a monoclonal antibody drug approved for the treatment of patients with lymphomas. Rituximab's main killing mechanism is antibody-dependent cellular cytotoxicity (ADCC). During ADCC, rituximab's fragment antigen binding (Fab) region binds to the CD20 antigen on the tumor cell and its fragment crystallizable (Fc) region binds to the Fc receptor (FcR) on the natural killer (NK) cells. In this study, two types of molecular interactions (CD20-rituximab, FcR-rituximab) involved in ADCC were measured simultaneously on cells prepared from biopsy specimens of lymphoma patients by utilizing atomic force microscopy (AFM) with functionalized tips carrying rituximab. NK cells were detected by specific NKp46 fluorescent labeling and tumor cells were detected by specific ROR1 fluorescent labeling. Based on the fluorescence recognition, the binding affinity and distribution of FcRs on NK cells, and CD20 on tumor cells, were quantitatively measured and mapped. The binding affinity and distribution of FcRs (on NK cells) and CD20 (on tumor cells) were associated with rituximab clinical efficacy. The experimental results provide a new approach to simultaneously quantify the multiple types of molecular interactions involved in rituximab ADCC mechanism on patient biopsy cells, which is of potential clinical significance to predict rituximab efficacy for personalized medicine. (C) 2014 Published by Elsevier Inc.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Cell Biology ; Immunology
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; NATURAL-KILLER-CELLS ; FC-GAMMA RECEPTORS ; ANTI-CD20 MONOCLONAL-ANTIBODIES ; SINGLE-MOLECULE ; LIVING CELLS ; CANCER-CELLS ; SURFACE EXPRESSION ; RECOGNITION EVENTS ; CLINICAL-EFFICACY
WOS研究方向Cell Biology ; Immunology
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/15268
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China
4.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China
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GB/T 7714
Li M,Xiao XB,Zhang WJ,et al. AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells[J]. CELLULAR IMMUNOLOGY,2014,290(2):233-244.
APA Li M,Xiao XB,Zhang WJ,Liu LQ,Xi N,&Wang YC.(2014).AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells.CELLULAR IMMUNOLOGY,290(2),233-244.
MLA Li M,et al."AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells".CELLULAR IMMUNOLOGY 290.2(2014):233-244.
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