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Regulating the mechanical properties of cells using a non-UV light-addressable hydrogel patterning process
Liu N(刘娜); Zhang CL(张常麟); Liu LQ(刘连庆); Wang YC(王越超); Gwo-Bin Lee; Li WJ(李文荣)
Department机器人学研究室
Conference Name2014 IEEE International Conference on Robotics and Automation (ICRA 2014)
Conference DateMay 31 - June 7, 2014
Conference PlaceHongKong, China
Source Publication2014 IEEE International Conference on Robotics and Automation (ICRA 2014)
PublisherIEEE
Publication PlacePiscataway, NJ, USA
2014
Pages5866-5870
Indexed ByEI ; CPCI(ISTP)
EI Accession number20152000853210
WOS IDWOS:000377221105136
Contribution Rank1
ISBN978-1-4799-3685-4
Abstract

The determination of the mechanical properties of cells plays an important role in biological studies and has gained acceptance recently as a possible label-free biomarker for cell status determination or diseases detection. Investigations on how external cellular properties affect cell mechanics are helpful in understanding cell disease processes and cell morphogenesis, which are of large significance in medical science. Although most researchers have focused on individual cell mechanics, or the effect of substrate stiffness on cells, cell mechanical response due to interactions among cells is yet to be examined. A reason for this is that the study of cell mechanical response to cell shape requires one to use a cell patterning process. However, existing cell patterning methods are very complex and time-consuming. In this paper, we describe a practical and rapid technique that can easily pattern cells into desired shapes, which allows investigations of the effect of external environment on cell stiffness. In the new technique, Poly-(ethylene) glycol diacrylate (PEGDA) hydrogel film with thickness 70–100 nm is controllably patterned on a hydrogenated amorphous silicon (a-Si:H) substrate by polymerizing PEGDA molecules in-situ using programmable visual light patterns. The idea is to enable the confinement of cells cultured on the hydrogels into special areas. The elastic modulus of the patterned cells is measured using an atomic force microscope. Experimental results have demonstrated the versatility of the technique as a tool for cell pattering and exploration of cell mechanics under external mechanical stimuli.

Language英语
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type会议论文
Identifierhttp://ir.sia.cn/handle/173321/15393
Collection机器人学研究室
Corresponding AuthorLiu N(刘娜)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences (CAS), China
2.Department of Power Mechanical Engineering, National Tsinghua University, Hsinchu, Taiwan
3.Dept. of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, Hong Kong
Recommended Citation
GB/T 7714
Liu N,Zhang CL,Liu LQ,et al. Regulating the mechanical properties of cells using a non-UV light-addressable hydrogel patterning process[C]. Piscataway, NJ, USA:IEEE,2014:5866-5870.
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