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Distinctive translational and self-rotational motion of lymphoma cells in an optically induced non-rotational alternating current electric field
Liang WF(梁文峰); Zhang, Ke; Yang, Xieliu; Liu LQ(刘连庆); Yu HB(于海波); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationBiomicrofluidics
ISSN1932-1058
2015
Volume9Issue:1Pages:1-15
Indexed BySCI ; EI
EI Accession number20150900567605
WOS IDWOS:000350546900021
Contribution Rank1
AbstractIn this paper, the translational motion and self-rotational behaviors of the Raji cells, a type of B-cell lymphoma cell, in an optically induced, non-rotational, electric field have been characterized by utilizing a digitally programmable and optically activated microfluidics chip with the assistance of an externally applied AC bias potential. The crossover frequency spectrum of the Raji cells was studied by observing the different linear translation responses of these cells to the positive and negative optically induced dielectrophoresis force generated by a projected light pattern. This digitally projected spot served as the virtual electrode to generate an axisymmetric and non-uniform electric field. Then, the membrane capacitance of the Raji cells could be directly measured. Furthermore, Raji cells under this condition also exhibited a self-rotation behavior. The repeatable and controlled self-rotation speeds of the Raji cells to the externally applied frequency and voltage were systematically investigated and characterized via computer-vision algorithms. The self-rotational speed of the Raji cells reached a maximum value at 60?kHz and demonstrated a quadratic relationship with respect to the applied voltage. Furthermore, optically projected patterns of four orthogonal electrodes were also employed as the virtual electrodes to manipulate the Raji cells. These results demonstrated that Raji cells located at the center of the four electrode pattern could not be self-rotated. Instead any Raji cells that deviated from this center area would also self-rotate. Most importantly, the Raji cells did not exhibit the self-rotational behavior after translating and rotating with respect to the center of any two adjacent electrodes. The spatial distributions of the electric field generated by the optically projected spot and the pattern of four electrodes were also modeled using a finite element numerical simulation. These simulations validated that the electric field distributions were non-uniform and non-rotational. Hence, the non-uniform electric field must play a key role in the self-rotation of the Raji cells. As a whole, this study elucidates an optoelectric-coupled microfluidics-based mechanism for cellular translation and self-rotation that can be used to extract the dielectric properties of the cells without using conventional metal-based microelectrodes. This technique may provide a simpler method for label-free identification of cancerous cells with many associated clinical applications. © 2015 AIP Publishing LLC.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas
WOS KeywordSINGLE CELLS ; DIELECTROPHORESIS ; ELECTROROTATION ; MANIPULATION ; ELECTROKINETICS ; IDENTIFICATION ; PROGRESSION ; PARTICLES ; MOLECULES ; BEHAVIOR
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/15756
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆)
Affiliation1.School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
3.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Liang WF,Zhang, Ke,Yang, Xieliu,et al. Distinctive translational and self-rotational motion of lymphoma cells in an optically induced non-rotational alternating current electric field[J]. Biomicrofluidics,2015,9(1):1-15.
APA Liang WF,Zhang, Ke,Yang, Xieliu,Liu LQ,Yu HB,&Zhang WJ.(2015).Distinctive translational and self-rotational motion of lymphoma cells in an optically induced non-rotational alternating current electric field.Biomicrofluidics,9(1),1-15.
MLA Liang WF,et al."Distinctive translational and self-rotational motion of lymphoma cells in an optically induced non-rotational alternating current electric field".Biomicrofluidics 9.1(2015):1-15.
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