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Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Antibody Atomic Force Microscopy Cell Complement-mediated Cytotoxicity Lymphoma Mechanical Properties
发表期刊IEEE Transactions on Nanobioscience
ISSN1536-1241
2015
卷号14期号:1页码:84-94
收录类别SCI ; EI
EI收录号20151000613084
WOS记录号WOS:000350884300009
产权排序1
资助机构National Natural Science Foundation of China (61175103, 61327014 and 61433017); the Research Fund of the State Key Laboratory of Robotics (No. 2014-Z07) and CAS FEA International Partnership Program for Creative Research Teams
摘要In the antibody-based targeted therapies of B-cell lymphomas, complement-mediated cytotoxicity (CMC) is an important mechanism. CMC is activated after the binding of drugs (monoclonal antibodies) to tumor cells. The activation of CMC ultimately leads to the lysis of tumor cells. However, it remains poorly understood how CMC alters the morphology and mechanics of single tumor cells at the nanoscale. In recent years, nanoscopic observations of cellular behaviors with the use of atomic force microscopy (AFM) have contributed much to the field of cell biology. In this work, by combining AFM with fluorescence microscopy, the detailed changes in cellular ultra-microstructures and mechanical properties during the process of CMC were quantitatively investigated on single tumor cells. AFM imaging distinctly showed that the CMC effect could lead to the formation of nano holes on the tumor cells. Quantitative analysis of AFM images indicated that cell surface became lower and rougher after the CMC process. The cellular mechanics measurements showed that during the process of CMC cells firstly softened and finally stiffened, which was validated by dynamically monitoring the mechanical changes of single living cells during CMC. The experimental results provide novel insights into the antibody-dependent CMC.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biochemical Research Methods ; Nanoscience & Nanotechnology
关键词[WOS]MONOCLONAL-ANTIBODIES ; MEMBRANE-ATTACK ; MECHANICAL-PROPERTIES ; LIVING CELLS ; CANCER-CELLS ; AFM ; HETEROGENEITY ; RITUXIMAB ; STIFFNESS ; MEDICINE
WOS研究方向Biochemistry & Molecular Biology ; Science & Technology - Other Topics
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/15777
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong
4.Department of Lymphoma, Hospital of Military Medical Academy of Sciences, Beijing, China
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GB/T 7714
Li M,Liu LQ,Xi N,et al. Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy[J]. IEEE Transactions on Nanobioscience,2015,14(1):84-94.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2015).Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy.IEEE Transactions on Nanobioscience,14(1),84-94.
MLA Li M,et al."Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy".IEEE Transactions on Nanobioscience 14.1(2015):84-94.
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