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Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationIEEE Transactions on Nanobioscience
ISSN1536-1241
2015
Volume14Issue:1Pages:84-94
Indexed BySCI ; EI
EI Accession number20151000613084
WOS IDWOS:000350884300009
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (61175103, 61327014 and 61433017); the Research Fund of the State Key Laboratory of Robotics (No. 2014-Z07) and CAS FEA International Partnership Program for Creative Research Teams
KeywordAntibody Atomic Force Microscopy Cell Complement-mediated Cytotoxicity Lymphoma Mechanical Properties
AbstractIn the antibody-based targeted therapies of B-cell lymphomas, complement-mediated cytotoxicity (CMC) is an important mechanism. CMC is activated after the binding of drugs (monoclonal antibodies) to tumor cells. The activation of CMC ultimately leads to the lysis of tumor cells. However, it remains poorly understood how CMC alters the morphology and mechanics of single tumor cells at the nanoscale. In recent years, nanoscopic observations of cellular behaviors with the use of atomic force microscopy (AFM) have contributed much to the field of cell biology. In this work, by combining AFM with fluorescence microscopy, the detailed changes in cellular ultra-microstructures and mechanical properties during the process of CMC were quantitatively investigated on single tumor cells. AFM imaging distinctly showed that the CMC effect could lead to the formation of nano holes on the tumor cells. Quantitative analysis of AFM images indicated that cell surface became lower and rougher after the CMC process. The cellular mechanics measurements showed that during the process of CMC cells firstly softened and finally stiffened, which was validated by dynamically monitoring the mechanical changes of single living cells during CMC. The experimental results provide novel insights into the antibody-dependent CMC.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Nanoscience & Nanotechnology
WOS KeywordMONOCLONAL-ANTIBODIES ; MEMBRANE-ATTACK ; MECHANICAL-PROPERTIES ; LIVING CELLS ; CANCER-CELLS ; AFM ; HETEROGENEITY ; RITUXIMAB ; STIFFNESS ; MEDICINE
WOS Research AreaBiochemistry & Molecular Biology ; Science & Technology - Other Topics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/15777
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong
4.Department of Lymphoma, Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy[J]. IEEE Transactions on Nanobioscience,2015,14(1):84-94.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2015).Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy.IEEE Transactions on Nanobioscience,14(1),84-94.
MLA Li M,et al."Quantitative analysis of drug-induced complement-mediated cytotoxic effect on single tumor cells using atomic force microscopy and fluorescence microscopy".IEEE Transactions on Nanobioscience 14.1(2015):84-94.
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