Superparamagnetic and surface-modified Fe3O4 nanoparticles were synthesized via high-temperature hydrolysis method. With the Fe3O4 nanoparticles as seed, silica stick grows on one side of nanoparticle via a non-classic controlled nucleation and growth method, and Janus-magnetic-mesoporous nanoparticles were achieved. The length of silica nanostick in Janus nanoparticle can be controlled by the amount of added TEOS. But the length of silica nanostick in primary growth process is limited, a secondary growth is important to increase the length of silica nanostick. Due to the mesoporous characteristic of silica nanostick, a loading and releasing experiment was conducted, and a sustained-releasing process of doxorubicin was verified.