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题名: Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope
作者: Zhang, Tianbiao ; Zhang CL(张常麟) ; Dong ZL(董再励) ; Guan, Yifu
作者部门: 机器人学研究室
刊名: SCIENTIFIC REPORTS
ISSN号: 2045-2322
出版日期: 2015
卷号: 5, 页码:1-7
收录类别: SCI
产权排序: 2
项目资助者: National Natural Science Foundation of China
摘要: As one of the most crucial properties of DNA, the structural stability and the mechanical strength are attracting a great attention. Here, we take advantage of high force resolution and high special resolution of Atom Force Microscope and investigate the mechanical force of DNA duplexes. To evaluate the base pair hydrogen bond strength and base stacking force in DNA strands, we designed two modes (unzipping and stretching) for the measurement rupture forces. Employing k-means clustering algorithm, the ruptured force are clustered and the mean values are estimated. We assessed the influence of experimental parameters and performed the force evaluation for DNA duplexes of pure dG/dC and dA/dT base pairs. The base binding strength of single dG/dC and single dA/dT were estimated to be 20.0 +/- 0.2 pN and 14.0 +/- 0.3 pN, respectively, and the base stacking interaction was estimated to be 2.0 +/- 0.1 pN. Our results provide valuable information about the quantitative evaluation of the mechanical properties of the DNA duplexes.
语种: 英语
WOS记录号: WOS:000351181000007
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
关键词[WOS]: SINGLE-MOLECULE ; COMPLEMENTARY STRANDS ; OPTICAL TWEEZERS ; MAGNETIC BEADS ; DOUBLE HELIX ; STABILITY ; SPECTROSCOPY ; OLIGONUCLEOTIDE ; ELASTICITY ; RESOLUTION
研究领域[WOS]: Science & Technology - Other Topics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/16226
Appears in Collections:机器人学研究室_期刊论文

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