SIA OpenIR  > 机器人学研究室
Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope
Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励); Guan, Yifu
作者部门机器人学研究室
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
2015
卷号5页码:1-7
收录类别SCI
WOS记录号WOS:000351181000007
产权排序2
资助机构National Natural Science Foundation of China
摘要As one of the most crucial properties of DNA, the structural stability and the mechanical strength are attracting a great attention. Here, we take advantage of high force resolution and high special resolution of Atom Force Microscope and investigate the mechanical force of DNA duplexes. To evaluate the base pair hydrogen bond strength and base stacking force in DNA strands, we designed two modes (unzipping and stretching) for the measurement rupture forces. Employing k-means clustering algorithm, the ruptured force are clustered and the mean values are estimated. We assessed the influence of experimental parameters and performed the force evaluation for DNA duplexes of pure dG/dC and dA/dT base pairs. The base binding strength of single dG/dC and single dA/dT were estimated to be 20.0 +/- 0.2 pN and 14.0 +/- 0.3 pN, respectively, and the base stacking interaction was estimated to be 2.0 +/- 0.1 pN. Our results provide valuable information about the quantitative evaluation of the mechanical properties of the DNA duplexes.
语种英语
WOS标题词Science & Technology
WOS类目Multidisciplinary Sciences
关键词[WOS]SINGLE-MOLECULE ; COMPLEMENTARY STRANDS ; OPTICAL TWEEZERS ; MAGNETIC BEADS ; DOUBLE HELIX ; STABILITY ; SPECTROSCOPY ; OLIGONUCLEOTIDE ; ELASTICITY ; RESOLUTION
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/16226
专题机器人学研究室
通讯作者Guan, Yifu
作者单位1.Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China. 110001
2.State Key Laboratory of Robotics, Shenyang Institute of Automatics, Chinese Academy of Sciences, Shenyang, China. 110016
推荐引用方式
GB/T 7714
Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope[J]. SCIENTIFIC REPORTS,2015,5:1-7.
APA Zhang, Tianbiao,Zhang CL,Dong ZL,&Guan, Yifu.(2015).Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope.SCIENTIFIC REPORTS,5,1-7.
MLA Zhang, Tianbiao,et al."Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope".SCIENTIFIC REPORTS 5(2015):1-7.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Determination of Bas(540KB)期刊论文出版稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Tianbiao]的文章
[Zhang CL(张常麟)]的文章
[Dong ZL(董再励)]的文章
百度学术
百度学术中相似的文章
[Zhang, Tianbiao]的文章
[Zhang CL(张常麟)]的文章
[Dong ZL(董再励)]的文章
必应学术
必应学术中相似的文章
[Zhang, Tianbiao]的文章
[Zhang CL(张常麟)]的文章
[Dong ZL(董再励)]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。