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Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope
Zhang, Tianbiao; Zhang CL(张常麟); Dong ZL(董再励); Guan, Yifu
Department机器人学研究室
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
2015
Volume5Pages:1-7
Indexed BySCI
WOS IDWOS:000351181000007
Contribution Rank2
Funding OrganizationNational Natural Science Foundation of China
AbstractAs one of the most crucial properties of DNA, the structural stability and the mechanical strength are attracting a great attention. Here, we take advantage of high force resolution and high special resolution of Atom Force Microscope and investigate the mechanical force of DNA duplexes. To evaluate the base pair hydrogen bond strength and base stacking force in DNA strands, we designed two modes (unzipping and stretching) for the measurement rupture forces. Employing k-means clustering algorithm, the ruptured force are clustered and the mean values are estimated. We assessed the influence of experimental parameters and performed the force evaluation for DNA duplexes of pure dG/dC and dA/dT base pairs. The base binding strength of single dG/dC and single dA/dT were estimated to be 20.0 +/- 0.2 pN and 14.0 +/- 0.3 pN, respectively, and the base stacking interaction was estimated to be 2.0 +/- 0.1 pN. Our results provide valuable information about the quantitative evaluation of the mechanical properties of the DNA duplexes.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordSINGLE-MOLECULE ; COMPLEMENTARY STRANDS ; OPTICAL TWEEZERS ; MAGNETIC BEADS ; DOUBLE HELIX ; STABILITY ; SPECTROSCOPY ; OLIGONUCLEOTIDE ; ELASTICITY ; RESOLUTION
WOS Research AreaScience & Technology - Other Topics
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/16226
Collection机器人学研究室
Corresponding AuthorGuan, Yifu
Affiliation1.Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, China. 110001
2.State Key Laboratory of Robotics, Shenyang Institute of Automatics, Chinese Academy of Sciences, Shenyang, China. 110016
Recommended Citation
GB/T 7714
Zhang, Tianbiao,Zhang CL,Dong ZL,et al. Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope[J]. SCIENTIFIC REPORTS,2015,5:1-7.
APA Zhang, Tianbiao,Zhang CL,Dong ZL,&Guan, Yifu.(2015).Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope.SCIENTIFIC REPORTS,5,1-7.
MLA Zhang, Tianbiao,et al."Determination of Base Binding Strength and Base Stacking Interaction of DNA Duplex Using Atomic Force Microscope".SCIENTIFIC REPORTS 5(2015):1-7.
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