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Super-resolution endoscopy for real-time wide-field imaging
Wang FF(王飞飞); Lai, Hok Sum Sam; Liu LQ(刘连庆); Li P(李盼); Yu HB(于海波); Liu Z(刘柱); Wang YC(王越超); Li WJ(李文荣)
作者部门机器人学研究室
发表期刊OPTICS EXPRESS
ISSN1094-4087
2015
卷号23期号:13页码:16803-16811
收录类别SCI ; EI
EI收录号20153701274669
WOS记录号WOS:000358543300032
产权排序1
资助机构NSFC/RGC Joint Research Scheme [51461165501, CityU132/14] ; CAS-Croucher Joint Lab Scheme [9500011] ; CAS FEA International Partnership Program for Creative Research Teams ; Hong Kong Research Grants Council [CityU 116912]
摘要Resolving subcellular structures in vitro beyond optical diffraction barrier by a light microscope has achieved significant development since the advancement of super-resolution fluorescence microscopes, such as stimulated emission depletion (STED) microscopy, stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM). However, the resolution of observation in deep and dense in vivo tissues is still confined to cellular level presently, and hence, exploring image details at subcellular level or even beyond organelle level in vivo has continued to attract much research attention. Currently, endoscopy provides an effective way to achieve in vivo observations and is compatible with mature optical microscopy technologies, but its resolution is usually confined to similar to 1 mu m. Here we report a new endoscopy method by functionalizing graded-index (GRIN) lens with microspheres for real-time white-light or fluorescent super-resolution imaging. The capability of resolving objects with feature size of similar to lambda/5, which breaks the diffraction barrier of traditional GRIN lens based endoscopes by a factor of two, has been demonstrated by using this superresolution endoscopy method. Further development of such a superresolution endoscopy technique may provide new opportunities for in vivo life sciences studies.
语种英语
WOS标题词Science & Technology ; Physical Sciences
WOS类目Optics
关键词[WOS]FLUORESCENCE MICROENDOSCOPY ; HIGH-SPEED ; MICROSCOPY ; MICROSPHERE ; RESOLUTION ; NANOSCOPE ; BREAKING ; PHOTON ; DEPTH ; LIMIT
WOS研究方向Optics
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/16853
专题机器人学研究室
通讯作者Li WJ(李文荣)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong, Hong Kong
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GB/T 7714
Wang FF,Lai, Hok Sum Sam,Liu LQ,et al. Super-resolution endoscopy for real-time wide-field imaging[J]. OPTICS EXPRESS,2015,23(13):16803-16811.
APA Wang FF.,Lai, Hok Sum Sam.,Liu LQ.,Li P.,Yu HB.,...&Li WJ.(2015).Super-resolution endoscopy for real-time wide-field imaging.OPTICS EXPRESS,23(13),16803-16811.
MLA Wang FF,et al."Super-resolution endoscopy for real-time wide-field imaging".OPTICS EXPRESS 23.13(2015):16803-16811.
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