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Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Mechanics Cancer Cell Temperature Cellular Interactions
发表期刊SCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
2015
卷号58期号:9页码:889-901
收录类别SCI ; CSCD
WOS记录号WOS:000362337500008
CSCD记录号CSCD:5530132
产权排序1
资助机构National Natural Science Foundation of China [61175103, 61375107, 61327014, 61433017] ; Research Fund of the State Key Laboratory of Robotics [2014-Z07] ; CAS FEA International Partnership Program for Creative Research Teams
摘要Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy (AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biology
关键词[WOS]BREAST-CANCER ; CYTOSKELETON ; ELASTICITY ; MEMBRANE ; ADHESION ; AFM ; BIOMECHANICS ; FIBROBLASTS ; GENERATION ; PROGRESS
WOS研究方向Life Sciences & Biomedicine - Other Topics
引用统计
被引频次:5[CSCD]   [CSCD记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/17126
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, USA
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
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GB/T 7714
Li M,Liu LQ,Xi N,et al. Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(9):889-901.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2015).Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy.SCIENCE CHINA-LIFE SCIENCES,58(9),889-901.
MLA Li M,et al."Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy".SCIENCE CHINA-LIFE SCIENCES 58.9(2015):889-901.
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