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题名: Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy
作者: Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: atomic force microscopy ; mechanics ; cancer cell ; temperature ; cellular interactions
刊名: SCIENCE CHINA-LIFE SCIENCES
ISSN号: 1674-7305
出版日期: 2015
卷号: 58, 期号:9, 页码:889-901
收录类别: SCI ; CSCD
产权排序: 1
项目资助者: National Natural Science Foundation of China [61175103, 61375107, 61327014, 61433017] ; Research Fund of the State Key Laboratory of Robotics [2014-Z07] ; CAS FEA International Partnership Program for Creative Research Teams
摘要: Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy (AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.
语种: 英语
WOS记录号: WOS:000362337500008
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
关键词[WOS]: BREAST-CANCER ; CYTOSKELETON ; ELASTICITY ; MEMBRANE ; ADHESION ; AFM ; BIOMECHANICS ; FIBROBLASTS ; GENERATION ; PROGRESS
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/17126
Appears in Collections:机器人学研究室_期刊论文

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