SIA OpenIR  > 机器人学研究室
Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Xiao XB(肖秀斌); Zhang WJ(张伟京)
Department机器人学研究室
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
2015
Volume58Issue:9Pages:889-901
Indexed BySCI ; CSCD
WOS IDWOS:000362337500008
CSCD IDCSCD:5530132
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China [61175103, 61375107, 61327014, 61433017] ; Research Fund of the State Key Laboratory of Robotics [2014-Z07] ; CAS FEA International Partnership Program for Creative Research Teams
KeywordAtomic Force Microscopy Mechanics Cancer Cell Temperature Cellular Interactions
AbstractCell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy (AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiology
WOS KeywordBREAST-CANCER ; CYTOSKELETON ; ELASTICITY ; MEMBRANE ; ADHESION ; AFM ; BIOMECHANICS ; FIBROBLASTS ; GENERATION ; PROGRESS
WOS Research AreaLife Sciences & Biomedicine - Other Topics
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Cited Times:6[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/17126
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, USA
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xi N,et al. Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(9):889-901.
APA Li M,Liu LQ,Xi N,Wang YC,Xiao XB,&Zhang WJ.(2015).Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy.SCIENCE CHINA-LIFE SCIENCES,58(9),889-901.
MLA Li M,et al."Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy".SCIENCE CHINA-LIFE SCIENCES 58.9(2015):889-901.
Files in This Item: Download All
File Name/Size DocType Version Access License
Effects of temperatu(3877KB)期刊论文出版稿开放获取ODC PDDLView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xi N(席宁)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xi N(席宁)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xi N(席宁)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.