Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels
作者: Yang WG(杨文广) ; Yu HB(于海波) ; Wei FN(魏发南); Li GX(李恭新); Wang YC(王越超) ; Liu LQ(刘连庆)
关键词: Cell pattern
刊名: Biomedical Microdevices
卷号: 17, 6, 1-8
项目资助者: National Natural Science Foundation of China (Project No. 61302003,61475183 and 61503258), the CAS FEA International Partnership Program for Creative Research Teams and the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. yz201339).
摘要: Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10 s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other. © 2015, Springer Science+Business Media New York.
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical
; Nanoscience & Nanotechnology
关键词[WOS]: ATOMIC-FORCE MICROSCOPY
; SELF-ASSEMBLED MONOLAYERS
; LIVING CELLS
; Science & Technology - Other Topics
Appears in Collections: 机器人学研究室_期刊论文
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Yang WG,Yu HB,Wei FN,et al. Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels[J]. Biomedical Microdevices,2015,17(6):1-8.