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Investigating the Molecular Specific Interactions on Cell Surface Using Atomic Force Microscopy
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超)
作者部门机器人学研究室
专著(文集)名Micro- and Nanomanipulation Tools
编著者Sun, Yu
出版者Wiley-VCH
出版地Weinheim, Germany
2015
ISBN号978-3-527-33784-2
出处页码417-440
收录类别EI
EI收录号20182705452462
摘要

Atomic force microscopy (AFM) offers an exciting tool for quantitatively investigating the physiological activities at single-cell and single-molecule levels. Here, we describe the applications of AFM single-molecule force spectroscopy (SMFS) in investigating the molecular specific interactions on the cell surface. This chapter begins with an introduction to the rituximab (an anti-CD20 antibody)–targeted therapy of lymphoma and discusses the challenges it faces. The following section presents a detailed description of SMFS, an important technique that enables AFM to locate and manipulate single molecules on the cell surface. Next, it presents how to measure the interaction forces between single CD20–rituximab pair directly on the tumor cells prepared from clinical lymphoma patients based on fluorescence recognition. Next, it presents how to map the nanoscale distribution of CD20 antigen on the tumor cells and analyze its potential role in the clinical treatments of rituximab. The nanoscale investigations of CD20–rituximab interactions on the surface of tumor cells using SMFS will be clinically useful for us to address the problem of resistance in the rituximab therapy of lymphomas.

语种英语
产权排序1
文献类型专著章节/文集论文
条目标识符http://ir.sia.cn/handle/173321/17497
专题机器人学研究室
通讯作者Li M(李密)
作者单位1.Shenyang Institute of Automation, Chinese Academy of Sciences
2.Department of Electrical and Computer Engineering, Michigan State University, 2 Lansing, USA
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Investigating the Molecular Specific Interactions on Cell Surface Using Atomic Force Microscopy. Micro- and Nanomanipulation Tools. Weinheim, Germany:Wiley-VCH,2015:417-440.
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