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Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties
Liu, Miao; Zheng, Mingjing; Xu, Hanying; Liu LQ(刘连庆); Li, Yanchun; Xiao, Wei; Li JC(李建春); Ma, Enlong
作者部门机器人学研究室
关键词Idiopathic Pulmonary Fibrosis (Ipf) Cyto-biophysical Properties Atomic Force Microscopy (Afm) Young's Modulus A549 Cells Salvianolic Acid b (Salb)
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
2015
卷号468期号:1-2页码:214-220
收录类别SCI
WOS记录号WOS:000366240800035
产权排序2
资助机构Educational Department of Liaoning Province [LJQ2013108] ; SPU Excellent Scholar Fund, Natural Science Foundation of Liaoning Province [2015020722] ; National Natural Science Foundation of China [81470135]
摘要Various methods have been used to evaluate anti-fibrotic activity of drugs. However, most of them are complicated, labor-intensive and lack of efficiency. This study was intended to develop a rapid method for anti-fibrotic drugs screening based on biophysical properties. A549 cells in vitro were stimulated with transforming growth factor-beta 1 (TGF-beta 1), and fibrogenesis was confirmed by conventional immunological assays. Meanwhile, the alterations of cyto-biophysical properties including morphology, roughness and stiffness were measured utilizing atomic force microscopy (AFM). It was found that fibrogenesis was accompanied with changes of cellular biophysical properties. TGF-beta 1-stimulated A549 cells became remarkably longer, rougher and stiffer than the control. Then, the effect of N-acetyl-L-cysteine (NAC) as a positive drug on ameliorating fibrogenesis in TGF-beta 1-stimulated A549 cells was verified respectively by immunological and biophysical markers. The result of Principal Component Analysis showed that stiffness was a leading index among all biophysical markers during fibrogenesis. Salvianolic acid B (SalB), a natural anti-oxidant, was detected by AFM to protect TGF-beta 1-stimulated A549 cells against stiffening. Then, SalB treatment was provided in preventive mode on a rat model of bleomycin (BLM) -induced pulmonary fibrosis. The results showed that SalB treatment significantly ameliorated BLM-induced histological alterations, blocked collagen accumulations and reduced alpha-SMA expression in lung tissues. All these results revealed the anti-pulmonary fibrotic activity of SalB. Detection of cyto-biophysical properties were therefore recommended as a rapid method for anti-pulmonary fibrotic drugs screening. (C) 2015 Elsevier Inc. All rights reserved.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; INTERMEDIATE-FILAMENTS ; CELLS ; FIBROSIS ; BIOMECHANICS ; CARCINOMA ; VIMENTIN ; CANCER ; AFM
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/17577
专题机器人学研究室
通讯作者Li JC(李建春); Ma, Enlong
作者单位1.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 110016, China
2.Shenyang Institute of Automation China Academy of Sciences, Shenyang, 110016, China
3.Jiangsu Kanion Pharmaceutical Co., Ltd., Nanjing, 222001, China
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GB/T 7714
Liu, Miao,Zheng, Mingjing,Xu, Hanying,et al. Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,468(1-2):214-220.
APA Liu, Miao.,Zheng, Mingjing.,Xu, Hanying.,Liu LQ.,Li, Yanchun.,...&Ma, Enlong.(2015).Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,468(1-2),214-220.
MLA Liu, Miao,et al."Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 468.1-2(2015):214-220.
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