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题名: Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties
作者: Liu, Miao; Zheng, Mingjing; Xu, Hanying; Liu LQ(刘连庆); Li, Yanchun; Xiao, Wei; Li JC(李建春); Ma, Enlong
作者部门: 机器人学研究室
关键词: Idiopathic pulmonary fibrosis (IPF) ; Cyto-biophysical properties ; Atomic force microscopy (AFM) ; Young's modulus ; A549 cells ; Salvianolic acid B (SalB)
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN号: 0006-291X
出版日期: 2015
卷号: 468, 期号:1-2, 页码:214-220
收录类别: SCI
产权排序: 2
项目资助者: Educational Department of Liaoning Province [LJQ2013108] ; SPU Excellent Scholar Fund, Natural Science Foundation of Liaoning Province [2015020722] ; National Natural Science Foundation of China [81470135]
摘要: Various methods have been used to evaluate anti-fibrotic activity of drugs. However, most of them are complicated, labor-intensive and lack of efficiency. This study was intended to develop a rapid method for anti-fibrotic drugs screening based on biophysical properties. A549 cells in vitro were stimulated with transforming growth factor-beta 1 (TGF-beta 1), and fibrogenesis was confirmed by conventional immunological assays. Meanwhile, the alterations of cyto-biophysical properties including morphology, roughness and stiffness were measured utilizing atomic force microscopy (AFM). It was found that fibrogenesis was accompanied with changes of cellular biophysical properties. TGF-beta 1-stimulated A549 cells became remarkably longer, rougher and stiffer than the control. Then, the effect of N-acetyl-L-cysteine (NAC) as a positive drug on ameliorating fibrogenesis in TGF-beta 1-stimulated A549 cells was verified respectively by immunological and biophysical markers. The result of Principal Component Analysis showed that stiffness was a leading index among all biophysical markers during fibrogenesis. Salvianolic acid B (SalB), a natural anti-oxidant, was detected by AFM to protect TGF-beta 1-stimulated A549 cells against stiffening. Then, SalB treatment was provided in preventive mode on a rat model of bleomycin (BLM) -induced pulmonary fibrosis. The results showed that SalB treatment significantly ameliorated BLM-induced histological alterations, blocked collagen accumulations and reduced alpha-SMA expression in lung tissues. All these results revealed the anti-pulmonary fibrotic activity of SalB. Detection of cyto-biophysical properties were therefore recommended as a rapid method for anti-pulmonary fibrotic drugs screening. (C) 2015 Elsevier Inc. All rights reserved.
语种: 英语
WOS记录号: WOS:000366240800035
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; INTERMEDIATE-FILAMENTS ; CELLS ; FIBROSIS ; BIOMECHANICS ; CARCINOMA ; VIMENTIN ; CANCER ; AFM
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/17577
Appears in Collections:机器人学研究室_期刊论文

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