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Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties
Liu, Miao; Zheng, Mingjing; Xu, Hanying; Liu LQ(刘连庆); Li, Yanchun; Xiao, Wei; Li JC(李建春); Ma, Enlong
Department机器人学研究室
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
2015
Volume468Issue:1-2Pages:214-220
Indexed BySCI
WOS IDWOS:000366240800035
Contribution Rank2
Funding OrganizationEducational Department of Liaoning Province [LJQ2013108] ; SPU Excellent Scholar Fund, Natural Science Foundation of Liaoning Province [2015020722] ; National Natural Science Foundation of China [81470135]
KeywordIdiopathic Pulmonary Fibrosis (Ipf) Cyto-biophysical Properties Atomic Force Microscopy (Afm) Young's Modulus A549 Cells Salvianolic Acid b (Salb)
AbstractVarious methods have been used to evaluate anti-fibrotic activity of drugs. However, most of them are complicated, labor-intensive and lack of efficiency. This study was intended to develop a rapid method for anti-fibrotic drugs screening based on biophysical properties. A549 cells in vitro were stimulated with transforming growth factor-beta 1 (TGF-beta 1), and fibrogenesis was confirmed by conventional immunological assays. Meanwhile, the alterations of cyto-biophysical properties including morphology, roughness and stiffness were measured utilizing atomic force microscopy (AFM). It was found that fibrogenesis was accompanied with changes of cellular biophysical properties. TGF-beta 1-stimulated A549 cells became remarkably longer, rougher and stiffer than the control. Then, the effect of N-acetyl-L-cysteine (NAC) as a positive drug on ameliorating fibrogenesis in TGF-beta 1-stimulated A549 cells was verified respectively by immunological and biophysical markers. The result of Principal Component Analysis showed that stiffness was a leading index among all biophysical markers during fibrogenesis. Salvianolic acid B (SalB), a natural anti-oxidant, was detected by AFM to protect TGF-beta 1-stimulated A549 cells against stiffening. Then, SalB treatment was provided in preventive mode on a rat model of bleomycin (BLM) -induced pulmonary fibrosis. The results showed that SalB treatment significantly ameliorated BLM-induced histological alterations, blocked collagen accumulations and reduced alpha-SMA expression in lung tissues. All these results revealed the anti-pulmonary fibrotic activity of SalB. Detection of cyto-biophysical properties were therefore recommended as a rapid method for anti-pulmonary fibrotic drugs screening. (C) 2015 Elsevier Inc. All rights reserved.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS KeywordATOMIC-FORCE MICROSCOPY ; INTERMEDIATE-FILAMENTS ; CELLS ; FIBROSIS ; BIOMECHANICS ; CARCINOMA ; VIMENTIN ; CANCER ; AFM
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/17577
Collection机器人学研究室
Corresponding AuthorLi JC(李建春); Ma, Enlong
Affiliation1.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 110016, China
2.Shenyang Institute of Automation China Academy of Sciences, Shenyang, 110016, China
3.Jiangsu Kanion Pharmaceutical Co., Ltd., Nanjing, 222001, China
Recommended Citation
GB/T 7714
Liu, Miao,Zheng, Mingjing,Xu, Hanying,et al. Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,468(1-2):214-220.
APA Liu, Miao.,Zheng, Mingjing.,Xu, Hanying.,Liu LQ.,Li, Yanchun.,...&Ma, Enlong.(2015).Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,468(1-2),214-220.
MLA Liu, Miao,et al."Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 468.1-2(2015):214-220.
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