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题名: 3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers
作者: Du, Mingchun; Chen B(陈冰); Meng QY(孟庆圆); Liu SM(刘素梅); Zheng XF(郑雄飞); Zhang C(张诚); Wang HR(王赫然); Li HY(李洪谊); Wang N(王诺); Dai JW(戴建武)
作者部门: 机器人学研究室
关键词: 3D bioprinting ; BMSC ; methacrylamide gelatin scaffold ; CBD-BMP2-collagen microfiber ; osteogenic differentiation
刊名: BIOFABRICATION
ISSN号: 1758-5082
出版日期: 2015
卷号: 7, 期号:4, 页码:1-10
收录类别: SCI ; EI
产权排序: 3
项目资助者: Research Equipment Development Program of the Chinese Academy of Sciences [YZ201351] ; National High Technology Research and Development Program of China (863 Program) [2012AA020501] ; National Natural Science Foundation of China [51305438] ; Ministry of Science and Technology of China (973 Program) [2011CB965001] ; 'Strategic Priority Research Program' of the Chinese Academy of Sciences [XDA01030000]
摘要: Three-dimensional (3D) bioprinting combines biomaterials, cells and functional components into complex living tissues. Herein, we assembled function-control modules into cell-laden scaffolds using 3D bioprinting. A customized 3D printer was able to tune the microstructure of printed bone mesenchymal stem cell (BMSC)-laden methacrylamide gelatin scaffolds at the micrometer scale. For example, the pore size was adjusted to 282 +/- 32 mu m and 363 +/- 60 mu m. To match the requirements of the printing nozzle, collagen microfibers with a length of 22 +/- 13 mu m were prepared with a highspeed crusher. Collagen microfibers bound bone morphogenetic protein 2 (BMP2) with a collagen binding domain (CBD) as differentiation-control module, from which BMP2 was able to be controllably released. The differentiation behaviors of BMSCs in the printed scaffolds were compared in three microenvironments: samples without CBD-BMP2-collagen microfibers in the growth medium, samples without microfibers in the osteogenic medium and samples with microfibers in the growth medium. The results indicated that BMSCs showed high cell viability (>90%) during printing; CBD-BMP2-collagen microfibers induced BMSC differentiation into osteocytes within 14 days more efficiently than the osteogenic medium. Our studies suggest that these function-control modules are attractive biomaterials and have potential applications in 3D bioprinting.
语种: 英语
WOS记录号: WOS:000366896900004
WOS标题词: Science & Technology ; Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
关键词[WOS]: MESENCHYMAL STEM-CELLS ; BONE MORPHOGENETIC PROTEIN-2 ; FIBROBLAST-GROWTH-FACTOR ; CROSS-LINKING HEPARIN ; COLLAGEN SCAFFOLDS ; EXTRACELLULAR-MATRIX ; ENGINEERED CARTILAGE ; CHONDROITIN SULFATE ; TISSUE ; REGENERATION
研究领域[WOS]: Engineering ; Materials Science
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/17578
Appears in Collections:机器人学研究室_期刊论文

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Recommended Citation:
Du, Mingchun,Chen B,Meng QY,et al. 3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers[J]. BIOFABRICATION,2015,7(4):1-10.
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