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3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers
Du, Mingchun; Chen B(陈冰); Meng QY(孟庆圆); Liu SM(刘素梅); Zheng XF(郑雄飞); Zhang C(张诚); Wang HR(王赫然); Li HY(李洪谊); Wang N(王诺); Dai JW(戴建武)
Department机器人学研究室
Source PublicationBIOFABRICATION
ISSN1758-5082
2015
Volume7Issue:4Pages:1-10
Indexed BySCI ; EI
EI Accession number20160301809986
WOS IDWOS:000366896900004
Contribution Rank3
Funding OrganizationResearch Equipment Development Program of the Chinese Academy of Sciences [YZ201351] ; National High Technology Research and Development Program of China (863 Program) [2012AA020501] ; National Natural Science Foundation of China [51305438] ; Ministry of Science and Technology of China (973 Program) [2011CB965001] ; 'Strategic Priority Research Program' of the Chinese Academy of Sciences [XDA01030000]
Keyword3d Bioprinting Bmsc Methacrylamide Gelatin Scaffold Cbd-bmp2-collagen Microfiber Osteogenic Differentiation
AbstractThree-dimensional (3D) bioprinting combines biomaterials, cells and functional components into complex living tissues. Herein, we assembled function-control modules into cell-laden scaffolds using 3D bioprinting. A customized 3D printer was able to tune the microstructure of printed bone mesenchymal stem cell (BMSC)-laden methacrylamide gelatin scaffolds at the micrometer scale. For example, the pore size was adjusted to 282 +/- 32 mu m and 363 +/- 60 mu m. To match the requirements of the printing nozzle, collagen microfibers with a length of 22 +/- 13 mu m were prepared with a highspeed crusher. Collagen microfibers bound bone morphogenetic protein 2 (BMP2) with a collagen binding domain (CBD) as differentiation-control module, from which BMP2 was able to be controllably released. The differentiation behaviors of BMSCs in the printed scaffolds were compared in three microenvironments: samples without CBD-BMP2-collagen microfibers in the growth medium, samples without microfibers in the osteogenic medium and samples with microfibers in the growth medium. The results indicated that BMSCs showed high cell viability (>90%) during printing; CBD-BMP2-collagen microfibers induced BMSC differentiation into osteocytes within 14 days more efficiently than the osteogenic medium. Our studies suggest that these function-control modules are attractive biomaterials and have potential applications in 3D bioprinting.
Language英语
WOS HeadingsScience & Technology ; Technology
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS KeywordMESENCHYMAL STEM-CELLS ; BONE MORPHOGENETIC PROTEIN-2 ; FIBROBLAST-GROWTH-FACTOR ; CROSS-LINKING HEPARIN ; COLLAGEN SCAFFOLDS ; EXTRACELLULAR-MATRIX ; ENGINEERED CARTILAGE ; CHONDROITIN SULFATE ; TISSUE ; REGENERATION
WOS Research AreaEngineering ; Materials Science
Citation statistics
Cited Times:71[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/17578
Collection机器人学研究室
Corresponding AuthorDu, Mingchun
Affiliation1.State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
2.ZonHon Biopharma Institute, Inc., Changzhou, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
Recommended Citation
GB/T 7714
Du, Mingchun,Chen B,Meng QY,et al. 3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers[J]. BIOFABRICATION,2015,7(4):1-10.
APA Du, Mingchun.,Chen B.,Meng QY.,Liu SM.,Zheng XF.,...&Dai JW.(2015).3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers.BIOFABRICATION,7(4),1-10.
MLA Du, Mingchun,et al."3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers".BIOFABRICATION 7.4(2015):1-10.
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