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题名: Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening
作者: Ma JY(马静云); Zhang, Xu; Liu Y(刘扬); Yu HB(于海波); Liu LQ(刘连庆); Shi Y(石杨); Li YF(李艳峰); Qin JH(秦建华)
作者部门: 机器人学研究室
关键词: Drug screening ; Hypoxia-inducible factors (HIFs) ; Micro-patterning ; Multicellular spheroids ; Phenotype transition
刊名: Biotechnology Journal
ISSN号: 1860-6768
出版日期: 2016
卷号: 11, 期号:1, 页码:127-134
收录类别: SCI ; EI
产权排序: 1
项目资助者: International Science & Technology Cooperation Program of China (No. 2015DFA00740), Key Laboratory of Separation Science for Analytical Chemistry (Dalian Institute of Chemical Physics, Chinese Academy of Sciences), National Nature Science Foundation of China (No. 81201689, 31171149).
摘要: 3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.
语种: 英语
WOS记录号: WOS:000370002300012
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology
关键词[WOS]: CELL-CULTURE SYSTEMS ; IN-VITRO ; MESENCHYMAL TRANSITION ; CANCER ; MODELS ; GROWTH ; IDENTIFICATION ; DISCOVERY ; INVASION ; 3-D
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/17615
Appears in Collections:机器人学研究室_期刊论文

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Recommended Citation:
Ma JY,Zhang, Xu,Liu Y,et al. Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening[J]. Biotechnology Journal,2016,11(1):127-134.
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