SIA OpenIR  > 机器人学研究室
Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening
Ma JY(马静云); Zhang, Xu; Liu Y(刘扬); Yu HB(于海波); Liu LQ(刘连庆); Shi Y(石杨); Li YF(李艳峰); Qin JH(秦建华)
作者部门机器人学研究室
关键词Drug Screening Hypoxia-inducible Factors (Hifs) Micro-patterning Multicellular Spheroids Phenotype Transition
发表期刊Biotechnology Journal
ISSN1860-6768
2016
卷号11期号:1页码:127-134
收录类别SCI ; EI
EI收录号20160101763105
WOS记录号WOS:000370002300012
产权排序1
资助机构International Science & Technology Cooperation Program of China (No. 2015DFA00740), Key Laboratory of Separation Science for Analytical Chemistry (Dalian Institute of Chemical Physics, Chinese Academy of Sciences), National Nature Science Foundation of China (No. 81201689, 31171149).
摘要3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.
语种英语
WOS标题词Science & Technology ; Life Sciences & Biomedicine
WOS类目Biochemical Research Methods ; Biotechnology & Applied Microbiology
关键词[WOS]CELL-CULTURE SYSTEMS ; IN-VITRO ; MESENCHYMAL TRANSITION ; CANCER ; MODELS ; GROWTH ; IDENTIFICATION ; DISCOVERY ; INVASION ; 3-D
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/17615
专题机器人学研究室
通讯作者Qin JH(秦建华)
作者单位1.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
2.The First Affiliated Hospital of Dalian Medical University, Dalian, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
推荐引用方式
GB/T 7714
Ma JY,Zhang, Xu,Liu Y,et al. Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening[J]. Biotechnology Journal,2016,11(1):127-134.
APA Ma JY.,Zhang, Xu.,Liu Y.,Yu HB.,Liu LQ.,...&Qin JH.(2016).Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.Biotechnology Journal,11(1),127-134.
MLA Ma JY,et al."Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening".Biotechnology Journal 11.1(2016):127-134.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Patterning hypoxic m(2425KB)期刊论文作者接受稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma JY(马静云)]的文章
[Zhang, Xu]的文章
[Liu Y(刘扬)]的文章
百度学术
百度学术中相似的文章
[Ma JY(马静云)]的文章
[Zhang, Xu]的文章
[Liu Y(刘扬)]的文章
必应学术
必应学术中相似的文章
[Ma JY(马静云)]的文章
[Zhang, Xu]的文章
[Liu Y(刘扬)]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。