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Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening
Ma JY(马静云); Zhang, Xu; Liu Y(刘扬); Yu HB(于海波); Liu LQ(刘连庆); Shi Y(石杨); Li YF(李艳峰); Qin JH(秦建华)
Department机器人学研究室
Source PublicationBiotechnology Journal
ISSN1860-6768
2016
Volume11Issue:1Pages:127-134
Indexed BySCI ; EI
EI Accession number20160101763105
WOS IDWOS:000370002300012
Contribution Rank1
Funding OrganizationInternational Science & Technology Cooperation Program of China (No. 2015DFA00740), Key Laboratory of Separation Science for Analytical Chemistry (Dalian Institute of Chemical Physics, Chinese Academy of Sciences), National Nature Science Foundation of China (No. 81201689, 31171149).
KeywordDrug Screening Hypoxia-inducible Factors (Hifs) Micro-patterning Multicellular Spheroids Phenotype Transition
Abstract3D multicellular spheroid models are of great value in the investigation of tumor biology and tumor responses to chemotherapy and radiation. To establish a mimicking tumor microenvironment in vitro, we developed a straightforward method by patterning hypoxic multicellular spheroids in a 3D matrix. The efficacy of this approach was evaluated by characterizing spheroid formation, invasive capability and phenotypic transition in aggressive human glioma cells. We observed enhanced cell proliferation, spheroid formation and invasive capability in U87 glioma cells transfected with hypoxia-inducible factors (HIFs) compared with non-treated cells. We also demonstrated that the overexpression of HIFs in hypoxic glioma cells may promote cell migration by epithelial-mesenchymal transition within the 3D matrix. Compared with conventional 3D culturing techniques, the simple operation, rapid prototyping, low cost and high throughput format of the micro-patterning method facilitates the characterization of cell proliferation, migration, phenotypic function and drug evaluation in physiologically relevant 3D microenvironments. This in vitro 3D system can recapitulate the physiologically relevant tumor microenvironment and is a promising method for 3D anti-tumor drug screening and the identification of novel targets for tumor invasion and angiogenesis.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology
WOS KeywordCELL-CULTURE SYSTEMS ; IN-VITRO ; MESENCHYMAL TRANSITION ; CANCER ; MODELS ; GROWTH ; IDENTIFICATION ; DISCOVERY ; INVASION ; 3-D
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/17615
Collection机器人学研究室
Corresponding AuthorQin JH(秦建华)
Affiliation1.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
2.The First Affiliated Hospital of Dalian Medical University, Dalian, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
Recommended Citation
GB/T 7714
Ma JY,Zhang, Xu,Liu Y,et al. Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening[J]. Biotechnology Journal,2016,11(1):127-134.
APA Ma JY.,Zhang, Xu.,Liu Y.,Yu HB.,Liu LQ.,...&Qin JH.(2016).Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening.Biotechnology Journal,11(1),127-134.
MLA Ma JY,et al."Patterning hypoxic multicellular spheroids in a 3D matrix - a promising method for anti-tumor drug screening".Biotechnology Journal 11.1(2016):127-134.
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