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基于光诱导介电泳机械力的免标记细胞电特性获取方法
Alternative TitleMethod for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force
刘连庆; 梁文峰; 李文荣; 张伟京; 肖秀斌
Department机器人学研究室
Rights Holder中国科学院沈阳自动化研究所
Patent Agent沈阳科苑专利商标代理有限公司 21002
Country中国
Subtype发明
Status驳回
Abstract本发明涉及基于光诱导介电泳机械力的免标记细胞电特性获取方法,包括:将入射光投影到光电子镊芯片下表面;信号发生器发出交流电压的频率,作用于光电子镊芯片的导电层;细胞开始远离入射光运动;增加外加交流电压的频率,当某细胞静止时,此时的频率为该细胞的交越频率;继续增加外加交流电压的频率,该细胞朝入射光处运动;当所有细胞均进入入射光范围内,关闭入射光及外加交流电压;得到各细胞的交越频率和直径的关系;根据细胞膜电容与细胞直径的关系,得到各细胞膜电容。本发明无需制备特异性结构的金属微电极;可实时获取单个或多个细胞的电特性参数;无需利用抗体对细胞进行标识,具有自动化、批量化及吞吐量高的优点。
Other AbstractThe invention relates to a method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force. The method comprises the following steps: incident light is projected to a lower surface of a photoelectron tweezer chip; frequency of alternating voltage emitted by a signal-generator is affected on a conducting layer of the photoelectron tweezer chip; cells move far from the incident light; frequency of applied alternating voltage can be increased, when certain cell is stationary, the frequency is a cross-over frequency of the cell; frequency of the applied alternating voltage is continuously increased, the cell moves towards to the incident light; when all the cells move in an incident light scope, the incident light and the applied alternating voltage are closed; a relation between the cross-over frequency and a diameter of each cell can be obtained; and capacitance of each cell membrane can be obtained according to the relation between the cell membrane capacitance and cell diameter. The preparation of metal microelectrodes with a specific structure is not required; the electrical characteristic parameter of single cell or many cells can be obtained with real time; an antibody is not required for being used for identification of the cells, and the method has the advantages of automation, mass production and high throughput capacity.
PCT Attributes
Application Date2014-09-19
2016-04-13
Application NumberCN201410482823.2
Open (Notice) NumberCN105486867A
Language中文
Contribution Rank1
Document Type专利
Identifierhttp://ir.sia.cn/handle/173321/18568
Collection机器人学研究室
Affiliation中国科学院沈阳自动化研究所
Recommended Citation
GB/T 7714
刘连庆,梁文峰,李文荣,等. 基于光诱导介电泳机械力的免标记细胞电特性获取方法[P]. 2016-04-13.
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