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题名:
Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy
作者: Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超)
作者部门: 机器人学研究室
通讯作者: 刘连庆 ; 席宁
关键词: Atomic forcemicroscopy ; Viscoelastic properties ; Methotrexate ; Cell ; Young's modulus ; Relaxation time
刊名: Journal of Biological Physics
ISSN号: 0092-0606
出版日期: 2016
卷号: 42, 期号:2, 页码:551–569
收录类别: SCI
产权排序: 1
项目资助者: National Natural Science Foundation of China (61503372, 61522312, 61375107, 61327014, 61433017) and the CAS FEA International Partnership Program for Creative Research Teams.
摘要: Methotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation.
语种: 英语
WOS记录号: WOS:000385191200004
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biophysics
关键词[WOS]: CANCER-CELLS ; MECHANICAL-PROPERTIES ; TUMOR-CELLS ; CYTOSKELETON ; AFM ; DEFORMABILITY ; FLUORESCENCE ; RECOGNITION ; METASTASIS ; ELASTICITY
研究领域[WOS]: Biophysics
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/19238
Appears in Collections:机器人学研究室_期刊论文

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Recommended Citation:
Li M,Liu LQ,Xiao XB,et al. Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy[J]. Journal of Biological Physics,2016,42(2):551–569.
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