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Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy
Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超)
Department机器人学研究室
Source PublicationJournal of Biological Physics
ISSN0092-0606
2016
Volume42Issue:2Pages:551-569
Indexed BySCI
WOS IDWOS:000385191200004
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (61503372, 61522312, 61375107, 61327014, 61433017) and the CAS FEA International Partnership Program for Creative Research Teams.
KeywordAtomic Forcemicroscopy Viscoelastic Properties Methotrexate Cell Young's Modulus Relaxation Time
AbstractMethotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiophysics
WOS KeywordCANCER-CELLS ; MECHANICAL-PROPERTIES ; TUMOR-CELLS ; CYTOSKELETON ; AFM ; DEFORMABILITY ; FLUORESCENCE ; RECOGNITION ; METASTASIS ; ELASTICITY
WOS Research AreaBiophysics
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/19238
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, USA
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xiao XB,et al. Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy[J]. Journal of Biological Physics,2016,42(2):551-569.
APA Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2016).Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy.Journal of Biological Physics,42(2),551-569.
MLA Li M,et al."Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy".Journal of Biological Physics 42.2(2016):551-569.
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