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Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation
Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超)
作者部门机器人学研究室
关键词Atomic Force Microscopy Blood Cellular Viscoelastic Properties Lymphocyte Relaxation Time
发表期刊IEEE Transactions on Nanobioscience
ISSN1536-1241
2016
卷号15期号:5页码:398-411
收录类别SCI ; EI
EI收录号20164302927632
WOS记录号WOS:000385620900001
产权排序1
资助机构National Natural Science Foundation of China (61503372, 61522312, 61375107, 61327014, 61433017) and CAS FEA International Partnership Program for Creative Research Teams.
摘要Cell mechanics has been proved to be an effective biomarker for indicating cellular states. The advent of atomic force microscopy (AFM) provides an exciting instrument for measuring the mechanical properties of single cells. However, current AFM single-cell mechanical measurements are commonly performed on cell lines cultured in vitro which are quite different from the primary cells in the human body. Investigating the mechanical properties of primary cells from clinical environments can help us to better understand cell behaviors. Here, by combining AFM with magnetic beads cell isolation, the viscoelastic properties of human primary B lymphocytes were quantitatively measured. B lymphocytes were isolated from the peripheral blood of healthy volunteers by density gradient centrifugation and CD19 magnetic beads cell isolation. The activity and specificity of the isolated cells were confirmed by fluorescence microscopy. AFM imaging revealed the surface topography and geometric parameters of B lymphocytes. The instantaneous modulus and relaxation time of living B lymphocytes were measured by AFM indenting technique, showing that the instantaneous modulus of human normal B lymphocytes was 2-3 kPa and the relaxation times were 0.03-0.06 s and 0.35-0.55 s. The differences in cellular visocoelastic properties between primary B lymphocytes and cell lines cultured in vitro were analyzed. The study proves the capability of AFM in quantifying the viscoelastic properties of individual specific primary cells from the blood sample of clinical patients, which will improve our understanding of the behaviors of cells in the human body.
语种英语
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/19273
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Nanta Street 114#, Shenyang 110016, China
2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, United States
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Li M,Liu LQ,Xiao XB,et al. Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation[J]. IEEE Transactions on Nanobioscience,2016,15(5):398-411.
APA Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2016).Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation.IEEE Transactions on Nanobioscience,15(5),398-411.
MLA Li M,et al."Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation".IEEE Transactions on Nanobioscience 15.5(2016):398-411.
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