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Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation
Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超)
Department机器人学研究室
Source PublicationIEEE Transactions on Nanobioscience
ISSN1536-1241
2016
Volume15Issue:5Pages:398-411
Indexed BySCI ; EI
EI Accession number20164302927632
WOS IDWOS:000385620900001
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (61503372, 61522312, 61375107, 61327014, 61433017) and CAS FEA International Partnership Program for Creative Research Teams.
KeywordAtomic Force Microscopy Blood Cellular Viscoelastic Properties Lymphocyte Relaxation Time
AbstractCell mechanics has been proved to be an effective biomarker for indicating cellular states. The advent of atomic force microscopy (AFM) provides an exciting instrument for measuring the mechanical properties of single cells. However, current AFM single-cell mechanical measurements are commonly performed on cell lines cultured in vitro which are quite different from the primary cells in the human body. Investigating the mechanical properties of primary cells from clinical environments can help us to better understand cell behaviors. Here, by combining AFM with magnetic beads cell isolation, the viscoelastic properties of human primary B lymphocytes were quantitatively measured. B lymphocytes were isolated from the peripheral blood of healthy volunteers by density gradient centrifugation and CD19 magnetic beads cell isolation. The activity and specificity of the isolated cells were confirmed by fluorescence microscopy. AFM imaging revealed the surface topography and geometric parameters of B lymphocytes. The instantaneous modulus and relaxation time of living B lymphocytes were measured by AFM indenting technique, showing that the instantaneous modulus of human normal B lymphocytes was 2-3 kPa and the relaxation times were 0.03-0.06 s and 0.35-0.55 s. The differences in cellular visocoelastic properties between primary B lymphocytes and cell lines cultured in vitro were analyzed. The study proves the capability of AFM in quantifying the viscoelastic properties of individual specific primary cells from the blood sample of clinical patients, which will improve our understanding of the behaviors of cells in the human body.
Language英语
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/19273
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Nanta Street 114#, Shenyang 110016, China
2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, United States
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xiao XB,et al. Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation[J]. IEEE Transactions on Nanobioscience,2016,15(5):398-411.
APA Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2016).Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation.IEEE Transactions on Nanobioscience,15(5),398-411.
MLA Li M,et al."Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation".IEEE Transactions on Nanobioscience 15.5(2016):398-411.
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