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Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging
Wang FF(王飞飞); Liu LQ(刘连庆); Yu HB(于海波); Wen YD(文扬东); Yu P(于鹏); Liu Z(刘柱); Wang YC(王越超); Li WJ(李文荣)
作者部门机器人学研究室
发表期刊Nature Communications
ISSN2041-1723
2016
卷号7页码:1-10
收录类别SCI
WOS记录号WOS:000389537800001
产权排序1
摘要Nanoscale correlation of structural information acquisition with specific-molecule identification provides new insight for studying rare subcellular events. To achieve this correlation, scanning electron microscopy has been combined with super-resolution fluorescent microscopy, despite its destructivity when acquiring biological structure information. Here we propose time-efficient non-invasive microsphere-based scanning superlens microscopy that enables the large-area observation of live-cell morphology or sub-membrane structures with sub-diffraction-limited resolution and is demonstrated by observing biological and non-biological objects. This microscopy operates in both non-invasive and contact modes with B200 times the acquisition efficiency of atomic force microscopy, which is achieved by replacing the point of an atomic force microscope tip with an imaging area of microspheres and stitching the areas recorded during scanning, enabling sub-diffraction-limited resolution. Our method marks a possible path to non-invasive cell imaging and simultaneous tracking of specific molecules with nanoscale resolution, facilitating the study of subcellular events over a total cell period.
语种英语
WOS标题词Science & Technology
WOS类目Multidisciplinary Sciences
关键词[WOS]ATOMIC-FORCE MICROSCOPE ; MICROSPHERE OPTICAL NANOSCOPY ; ELECTRON-MICROSCOPY ; DIFFRACTION LIMIT ; SUPERRESOLUTION MICROSCOPY ; GRAPHENE NANORIBBONS ; RESOLUTION ; ARRAYS ; CELLS ; SPECTROSCOPY
WOS研究方向Science & Technology - Other Topics
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/19724
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Li WJ(李文荣)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon Tong 999077, Hong Kong
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GB/T 7714
Wang FF,Liu LQ,Yu HB,et al. Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging[J]. Nature Communications,2016,7:1-10.
APA Wang FF.,Liu LQ.,Yu HB.,Wen YD.,Yu P.,...&Li WJ.(2016).Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging.Nature Communications,7,1-10.
MLA Wang FF,et al."Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging".Nature Communications 7(2016):1-10.
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