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OEK-Based Dynamic Discrimination of B-lymphocyte Cells from Red Blood Cells
Liang WF(梁文峰); Liu B(刘斌); Liu LQ(刘连庆); Li WJ(李文荣); Zhang WJ(张伟京)
作者部门机器人学研究室
会议名称6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED)
会议日期November 04-07, 2012
会议地点Bangkok, THAILAND
会议主办者IEEE, Natl Nanotechnol Ctr, IEEE Nanotechnol Council, ETH Zurich, Univ Arizona, Johns Hopkins Univ, City Univ Hong Kong, Natl Elect & Comp Technol Ctr, Thailand Convent & Exhibit Bur
会议录名称6TH IEEE INTERNATIONAL CONFERENCE ON NANO/MOLECULAR MEDICINE AND ENGINEERING (IEEE-NANOMED 2012)
出版者IEEE
出版地NEW YORK
2012
页码1
收录类别CPCI(ISTP)
WOS记录号WOS:000322438500017
产权排序1
ISSN号2159-6964
ISBN号978-1-4673-5103-4
摘要

Exploring the clinicopathological characteristics of B-lymphocyte cells from human lymphoma patients is essential for cancer treatment. However, there are many red blood cells (RBCs) contained in the preparation sample of the B-lymphocyte cells. Thus, target of B-lymphocyte cells in the mixed cell populations are expected to be discriminated from RBCs that interfere with later experimental processes. Traditional cell separation methods, such as optical tweezers and dielectrophoresis (DEP), are commonly used to discriminate cells. However, the limits and drawbacks of those methods hamper their wider applications for cell discrimination. For example, the high optical power requirement for optical tweezers might kill live cells or influence the properties of cells due to the intense localized heating. Furthermore, DEP separation of cells requires the integrated micro pumps/valves, which require more complex fabrication procedures. In this talk, we report an approach to dynamically and spatially discriminate cultured human B-lymphocyte cells from normal red blood cells (RBCs) mixed in an isotonic solution, utilizing a hydrogenated, amorphous silicon (a-Si:H)-based optically-induced electrokinetics (OEK) chip. A theoretical calculation of Clausius-Mossotti factors for the two kinds of cells is first presented, which reflects the feasibility of the discrimination of the two kinds of cells via optically-induced dielectrophoresis (ODEP) technique based on the OEK chip. The composition of the isotonic solution in which the two kinds of cells is suspended is experimentally optimized, which can decrease the friction force between the cells and the a-Si:H substrate and enhance the ODEP manipulation and discrimination. The discrimination performance depends on the frequency and voltage of the AC source and the width of the incident optical image used as the virtual electrode for generating ODEP force.

语种英语
引用统计
文献类型会议论文
条目标识符http://ir.sia.cn/handle/173321/19876
专题机器人学研究室
通讯作者Liang WF(梁文峰)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
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Liang WF,Liu B,Liu LQ,et al. OEK-Based Dynamic Discrimination of B-lymphocyte Cells from Red Blood Cells[C]//IEEE, Natl Nanotechnol Ctr, IEEE Nanotechnol Council, ETH Zurich, Univ Arizona, Johns Hopkins Univ, City Univ Hong Kong, Natl Elect & Comp Technol Ctr, Thailand Convent & Exhibit Bur. NEW YORK:IEEE,2012:1.
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