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Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
Gu, Rui; Zhang X(张旭); Zhang, Ge; Tao, Tingting; Yu HB(于海波); Liu LQ(刘连庆); Dou Y(窦颖); Li AP(李爱萍); Qin JH(秦建华)
Department机器人学研究室
Source PublicationNeurochemical Research
ISSN0364-3190
2017
Volume42Issue:5Pages:1478-1487
Indexed BySCI
WOS IDWOS:000399906000021
Contribution Rank5
Funding OrganizationInternational Science & Technology Cooperation Program of China (No.2015DFA00740) ; the Instrument Developing Project of the Chinese Academy of Sciences, Key Laboratory of Separation Science for Analytical Chemistry (Dalian Institute of Chemical Physics, Chinese Academy of Sciences) ; Natural Science Foundation of Liaoning Province, China (No. 201202050) ; National Nature Science Foundation of China (No. 81402549)
KeywordMicrofluidic Device Microglia Glioma Epithelial-mesenchymal Transition Phagocytosis Migration
Abstract

It has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia.

Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology ; Neurosciences
WOS KeywordActivated Microglia ; Cells ; Transition ; Matrix ; Mechanisms ; Macrophage ; Resistant ; Migration ; Invasion ; System
WOS Research AreaBiochemistry & Molecular Biology ; Neurosciences & Neurology
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/20235
Collection机器人学研究室
Corresponding AuthorLi AP(李爱萍); Qin JH(秦建华)
Affiliation1.Department of Physiology, Dalian Medical University, 9 West Section, Lvshun South Road, Dalian, 116044, China
2.Center Laboratory Department, General Hospital of Chengdu Army, Chengdu, 610083, China
3.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457, Zhongshan Road, Dalian, 116023, China
4.State key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China
5.Department of Immunology, Dalian Medical University, 9 West Section, Lvshun South Road, Dalian, 116044, China.
Recommended Citation
GB/T 7714
Gu, Rui,Zhang X,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. Neurochemical Research,2017,42(5):1478-1487.
APA Gu, Rui.,Zhang X.,Zhang, Ge.,Tao, Tingting.,Yu HB.,...&Qin JH.(2017).Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice.Neurochemical Research,42(5),1478-1487.
MLA Gu, Rui,et al."Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice".Neurochemical Research 42.5(2017):1478-1487.
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