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Simultaneous Measurement of Multiple Mechanical Properties of Single Cells Using AFM by Indentation and Vibration
Zhang C(张闯); Shi JL(施佳林); Wang WX(王文学); Xi N(席宁); Wang YC(王越超); Liu LQ(刘连庆)
Department机器人学研究室
Source PublicationIEEE Transactions on Biomedical Engineering
ISSN0018-9294
2017
Volume64Issue:12Pages:2771-2780
Indexed BySCI ; EI
EI Accession number20180304651253
WOS IDWOS:000417722600003
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China ; CAS/SAFEA International Partnership Program for Creative Research Teams
KeywordAtomic Force Microscope (Afm) Cellular Biophysics Cellular Mechanical Property Indentation Vibrations Viscoelasticity
AbstractObjective: The mechanical properties of cells, which are the main characteristics determining their physical performance and physiological functions, have been actively studied in the fields of cytobiology and biomedical engineering and for the development of medicines. In this study, an indentation-vibration-based method is proposed to simultaneously measure the mechanical properties of cells in situ, including cellular mass (m), elasticity (k) and viscosity (c). Methods: The proposed measurement method is implemented based on the principle of forced vibration stimulated by simple harmonic force using an atomic force microscope (AFM) system integrated with a piezoelectric transducer (PZT) as the substrate vibrator. The corresponding theoretical model containing the three mechanical properties is derived and used to perform simulations and calculations. Living and fixed human embryonic kidney 293 (HEK 293) cells were subjected to indentation and vibration to measure and compare their mechanical parameters and verify the proposed approach. Results: The results that the fixed sample cells are more viscous and elastic than the living sample cells and the measured mechanical properties of cell are consistent within, but not outside of the central region of the cell, are in accordance with previous studies. Conclusion: This work provides an approach to simultaneous measurement of the multiple mechanical properties of single cells using an integrated AFM system based on the principle force vibration and thickness-corrected Hertz model. Significance: This work should contribute to progress in biomedical engineering, cytobiology, medicine, early diagnosis, specific therapy and cell-powered robots.
Language英语
WOS SubjectEngineering, Biomedical
WOS KeywordATOMIC-FORCE MICROSCOPY ; CANCER-CELLS ; MICROMECHANICAL PROPERTIES ; VISCOELASTIC PROPERTIES ; ELASTIC-MODULI ; ADHERENT CELLS ; BREAST-CANCER ; LIVING CELLS ; STIFFNESS ; MEMBRANE
WOS Research AreaEngineering
Funding ProjectNational Natural Science Foundation of China[61673372] ; National Natural Science Foundation of China[61433017] ; National Natural Science Foundation of China[61327014] ; National Natural Science Foundation of China[61522312] ; CAS/SAFEA International Partnership Program for Creative Research Teams
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/20238
Collection机器人学研究室
Corresponding AuthorWang WX(王文学); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences (CAS), University of the Chinese Academy of Sciences, Shenyang 110016, China
2.Emerging Technologies Institute, Department of Industrial and Manufacturing Systems Engineering, University of Hong Kong, Hong Kong
Recommended Citation
GB/T 7714
Zhang C,Shi JL,Wang WX,et al. Simultaneous Measurement of Multiple Mechanical Properties of Single Cells Using AFM by Indentation and Vibration[J]. IEEE Transactions on Biomedical Engineering,2017,64(12):2771-2780.
APA Zhang C,Shi JL,Wang WX,Xi N,Wang YC,&Liu LQ.(2017).Simultaneous Measurement of Multiple Mechanical Properties of Single Cells Using AFM by Indentation and Vibration.IEEE Transactions on Biomedical Engineering,64(12),2771-2780.
MLA Zhang C,et al."Simultaneous Measurement of Multiple Mechanical Properties of Single Cells Using AFM by Indentation and Vibration".IEEE Transactions on Biomedical Engineering 64.12(2017):2771-2780.
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