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The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy
Alternative Title基于AFM的化疗药物与质粒DNA间动态相互作用观测
Li M(李密)1; Liu LQ(刘连庆)1; Xiao XB(肖秀斌)2; Xi N(席宁)1,3; Wang YC(王越超)1
Department机器人学研究室
Source PublicationScience China Materials
ISSN2095-8226
2017
Volume60Issue:3Pages:269-278
Indexed BySCI ; EI ; CSCD
EI Accession number20174804469089
WOS IDWOS:000398129000008
CSCD IDCSCD:5931839
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (61503372, 61522312, 61327014 and 61433017) ; Youth Innovation Promotion Association CAS ; CAS FEA International Partnership Program for Creative Research Teams
KeywordAtomic Force Microscopy Plasmid Dna Drug Methotrexate Cisplatin
Abstract

The advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level.

Language英语
WOS HeadingsScience & Technology ; Technology
WOS SubjectMaterials Science, Multidisciplinary
WOS KeywordSingle-molecule ; Cancer-cells ; Measurements Reveal ; Persistence Length ; Membrane-proteins ; Spectroscopy ; Cisplatin ; Temperature ; Dependence ; Morphology
WOS Research AreaMaterials Science
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/20246
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Xi N(席宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, United States
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xiao XB,et al. The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy[J]. Science China Materials,2017,60(3):269-278.
APA Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2017).The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy.Science China Materials,60(3),269-278.
MLA Li M,et al."The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy".Science China Materials 60.3(2017):269-278.
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