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Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy
Li YC(李艳春); Chen, Jv; Liu, Yutong; Zhang, Weige; He, Wenhui; Xu, Hanying; Liu LQ(刘连庆); Ma EL(马恩龙)
Department机器人学研究室
Source PublicationPLOS ONE
ISSN1932-6203
2017
Volume12Issue:6Pages:1-16
Indexed BySCI
WOS IDWOS:000404043100016
Contribution Rank1
Funding OrganizationState Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences ; National Natural Science Foundation of China [81470135] ; Excellent Scholar Projects from the Educational Department of Liaoning Province [LJQ2013108] ; Natural Science Foundation of Liaoning Province [2015020722] ; SPU Excellent Scholar Fund
AbstractAs an inhibitor of microtubule assembly, combretastatin A-4 (CA-4)-induced biological responses in tumor cells have been well known, but the corresponding changes in nano-biophysical properties were not investigated given the lack of an ideal tool. Using AFM technique, we investigated the alteration of nano-biophysical properties when CA-4-treated tumor cells underwent the different biological processes, including cell cycle arrest, apoptosis and autophagy. We found that CA-4-resistant cells were rougher with the presence of characteristic "ridges", indicating that the development of "ridge" structure may be a determinant of the sensitivity of cells to CA-4 compounds. CA-4 induced G2/M arrest and apoptosis in sensitive cells but triggered anti-apoptotic autophagy in resistant cells. CA-4 treatment caused an increase in stiffness in both sensitive and resistant cells. However, these cells exhibited different changes in cell surface roughness. CA-4 decreased Ra and Rq values in sensitive cells but increased these values in resistant cells. The reorganization of F-actin might contribute to the different changes of nano-biophysical properties in CA-4-sensitive and-resistant cells. Our results suggest that cellular nano-biophysical properties, such as "ridges", roughness and stiffness, could be applied as potential biomarkers for evaluating CA-4 compounds, and knowledge regarding how biological alterations cause changes in cellular nano-biophysical properties is helpful to develop a new high-resolution screening tool for anti-tumor agents.
Language英语
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS KeywordAUTOPHAGY ; APOPTOSIS ; MECHANICS ; TUBULIN ; ARREST ; AGENT ; A-4
WOS Research AreaScience & Technology - Other Topics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/20804
Collection机器人学研究室
Corresponding AuthorMa EL(马恩龙)
Affiliation1.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences, Shenyang, China
3.Department of Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, China
Recommended Citation
GB/T 7714
Li YC,Chen, Jv,Liu, Yutong,et al. Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy[J]. PLOS ONE,2017,12(6):1-16.
APA Li YC.,Chen, Jv.,Liu, Yutong.,Zhang, Weige.,He, Wenhui.,...&Ma EL.(2017).Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy.PLOS ONE,12(6),1-16.
MLA Li YC,et al."Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy".PLOS ONE 12.6(2017):1-16.
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