SIA OpenIR  > 机器人学研究室
Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy
Li YC(李艳春); Chen, Jv; Liu, Yutong; Zhang, Weige; He, Wenhui; Xu, Hanying; Liu LQ(刘连庆); Ma EL(马恩龙)
作者部门机器人学研究室
发表期刊PLOS ONE
ISSN1932-6203
2017
卷号12期号:6页码:1-16
收录类别SCI
WOS记录号WOS:000404043100016
产权排序1
资助机构State Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences ; National Natural Science Foundation of China [81470135] ; Excellent Scholar Projects from the Educational Department of Liaoning Province [LJQ2013108] ; Natural Science Foundation of Liaoning Province [2015020722] ; SPU Excellent Scholar Fund
摘要As an inhibitor of microtubule assembly, combretastatin A-4 (CA-4)-induced biological responses in tumor cells have been well known, but the corresponding changes in nano-biophysical properties were not investigated given the lack of an ideal tool. Using AFM technique, we investigated the alteration of nano-biophysical properties when CA-4-treated tumor cells underwent the different biological processes, including cell cycle arrest, apoptosis and autophagy. We found that CA-4-resistant cells were rougher with the presence of characteristic "ridges", indicating that the development of "ridge" structure may be a determinant of the sensitivity of cells to CA-4 compounds. CA-4 induced G2/M arrest and apoptosis in sensitive cells but triggered anti-apoptotic autophagy in resistant cells. CA-4 treatment caused an increase in stiffness in both sensitive and resistant cells. However, these cells exhibited different changes in cell surface roughness. CA-4 decreased Ra and Rq values in sensitive cells but increased these values in resistant cells. The reorganization of F-actin might contribute to the different changes of nano-biophysical properties in CA-4-sensitive and-resistant cells. Our results suggest that cellular nano-biophysical properties, such as "ridges", roughness and stiffness, could be applied as potential biomarkers for evaluating CA-4 compounds, and knowledge regarding how biological alterations cause changes in cellular nano-biophysical properties is helpful to develop a new high-resolution screening tool for anti-tumor agents.
语种英语
WOS标题词Science & Technology
WOS类目Multidisciplinary Sciences
关键词[WOS]AUTOPHAGY ; APOPTOSIS ; MECHANICS ; TUBULIN ; ARREST ; AGENT ; A-4
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/20804
专题机器人学研究室
通讯作者Ma EL(马恩龙)
作者单位1.Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, China Academy of Sciences, Shenyang, China
3.Department of Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, China
推荐引用方式
GB/T 7714
Li YC,Chen, Jv,Liu, Yutong,et al. Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy[J]. PLOS ONE,2017,12(6):1-16.
APA Li YC.,Chen, Jv.,Liu, Yutong.,Zhang, Weige.,He, Wenhui.,...&Ma EL.(2017).Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy.PLOS ONE,12(6),1-16.
MLA Li YC,et al."Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy".PLOS ONE 12.6(2017):1-16.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Nanoscale quantifica(46206KB)期刊论文作者接受稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li YC(李艳春)]的文章
[Chen, Jv]的文章
[Liu, Yutong]的文章
百度学术
百度学术中相似的文章
[Li YC(李艳春)]的文章
[Chen, Jv]的文章
[Liu, Yutong]的文章
必应学术
必应学术中相似的文章
[Li YC(李艳春)]的文章
[Chen, Jv]的文章
[Liu, Yutong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Nanoscale quantification of the biophysical characterization of combretastatin A-4-treated tumor cells using atomic force microscopy.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。