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Accurate extraction of the self-rotational speed for cells in an electrokinetics force field by an image matching algorithm
Yang, Xieliu; Niu, Xihui; Liu Z(刘柱); Zhao, Yuliang; Zhang, Guanglie; Liang WF(梁文峰); Li WJ(李文荣)
Department机器人学研究室
Source PublicationMicromachines
ISSN2072-666X
2017
Volume8Issue:9Pages:1-17
Indexed BySCI ; EI
EI Accession number20173904205248
WOS IDWOS:000411523200024
Contribution Rank2
Funding OrganizationNational Natural Science Foundation of China (61503258, 51405481), the Natural Science Foundation of Liaoning Province (2015020129, 201602614), Scientific, the Technical Research Foundation of the Education Department of Liaoning Province (L2015438, L2015452), the Discipline Content Education Project (XKHY2-28), and the Hong Kong Research Grants Council (CityU 11215614).
KeywordCellular Behaviors Electrokinetics Field Image-tracking Self-rotation Template-matching
AbstractWe present an image-matching-based automated algorithm capable of accurately determining the self-rotational speed of cancer cells in an optically-induced electrokinetics-based microfluidic chip. To automatically track a specific cell in a video featuring more than one cell, a background subtraction technique was used. To determine the rotational speeds of cells, a reference frame was automatically selected and curve fitting was performed to improve the stability and accuracy. Results show that the algorithm was able to accurately calculate the self-rotational speeds of cells up to ∼150 rpm. In addition, the algorithm could be used to determine the motion trajectories of the cells. Potential applications for the developed algorithm include the differentiation of cell morphology and characterization of cell electrical properties.
Language英语
WOS HeadingsScience & Technology ; Technology
WOS SubjectNanoscience & Nanotechnology ; Instruments & Instrumentation
WOS KeywordHOLOGRAPHIC OPTICAL TWEEZERS ; DIELECTRIC-PROPERTIES ; ACOUSTIC-WAVES ; SINGLE CELLS ; LIVING CELLS ; TUMOR-CELLS ; ELECTROROTATION ; DIELECTROPHORESIS ; MANIPULATION ; PARTICLES
WOS Research AreaScience & Technology - Other Topics ; Instruments & Instrumentation
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/21006
Collection机器人学研究室
Corresponding AuthorLiang WF(梁文峰); Li WJ(李文荣)
Affiliation1.School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang, 110168, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 999077, Hong Kong
4.Institute of Advanced and Intelligent Sensing Systems, Shenzhen Academy of Robotics, Shenzhen, 518057, China
Recommended Citation
GB/T 7714
Yang, Xieliu,Niu, Xihui,Liu Z,et al. Accurate extraction of the self-rotational speed for cells in an electrokinetics force field by an image matching algorithm[J]. Micromachines,2017,8(9):1-17.
APA Yang, Xieliu.,Niu, Xihui.,Liu Z.,Zhao, Yuliang.,Zhang, Guanglie.,...&Li WJ.(2017).Accurate extraction of the self-rotational speed for cells in an electrokinetics force field by an image matching algorithm.Micromachines,8(9),1-17.
MLA Yang, Xieliu,et al."Accurate extraction of the self-rotational speed for cells in an electrokinetics force field by an image matching algorithm".Micromachines 8.9(2017):1-17.
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