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Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics
Liang WF(梁文峰); Zhao YL(赵玉良); Liu LQ(刘连庆); Wang YC(王越超); Li WJ(李文荣); Li GB(李国宾)
Department机器人学研究室
Source PublicationBIOPHYSICAL JOURNAL
ISSN0006-3495
2017
Volume113Issue:7Pages:1531-1539
Indexed BySCI
WOS IDWOS:000412551200016
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (NSFC) [61503258, 61522312, 61503322, 51405481] ; Natural Science Foundation of Liaoning Province [201602614] ; Hong Kong Research Grants Council (RGC) [CityU 125513] ; NSFC/RGC Joint Scheme [CityU132/14]
AbstractCell membrane capacitance and conductance are key pieces of intrinsic information correlated with the cellular dielectric parameters and morphology of the plasma membrane; these parameters have been used as electrophysiological biomarkers to characterize cellular phenotype and state, and they have many associated clinical applications. Here, we present our work on the non-invasive determination of cell membrane capacitance and conductance by an optically activated microfluidics chip. The model for determining the cell membrane capacitance and conductance was established by a single layer of the shellcore polarization model. Three-dimensional finite-element analyses of the positive and negative optically induced dielectrophoresis forces generated by the projected light arrays of spots were performed, thus providing a theoretical validation of the feasibility of this approach. Then, the crossover frequency spectra for four typical types of cells (Raji cells, MCF-7 cells, HEK293 cells, and K562 cells) were experimentally investigated by using a micro-vision based motion-tracking technique. The different responses of these cells to the positive and negative ODEP forces were studied under four different liquid conductivities by automatic observation and tracking of the cellular trajectory and texture during the cells' translation. The cell membrane capacitance and conductance were determined from the curve-fitted spectra, which were 11.1 +/- 0.9 mF/m(2) and 782 +/- 32 S/m(2), respectively, for Raji cells, 11.5 +/- 0.8 mF/m(2) and 114 +/- 28 S/m(2) for MCF-7 cells, 9.0 +/- 0.9 mF/m(2) and 187 +/- 22 S/m(2) for HEK293 cells, and 10.2 +/- 0.7 mF/m(2) and 879 +/- 24 S/m(2) for K562 cells. Furthermore, as an application of this technique, the membrane capacitances of MCF-7 cells treated with four different concentrations of drugs were acquired. This technique introduces a determination of cell membrane capacitance and conductance that yields statistically significant data while allowing information from individual cells to be obtained in a non-invasive manner.
Language英语
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiophysics
WOS KeywordELECTRIC-FIELD ; CANCER-CELLS ; TUMOR-CELLS ; DIELECTROPHORESIS ; ELECTROROTATION ; MANIPULATION ; CYTOPLASM ; FREQUENCY ; INTERPLAY ; EXHIBIT
WOS Research AreaBiophysics
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/21107
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆); Li WJ(李文荣)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang, China
3.Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong
4.School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao, China
5.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
Recommended Citation
GB/T 7714
Liang WF,Zhao YL,Liu LQ,et al. Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics[J]. BIOPHYSICAL JOURNAL,2017,113(7):1531-1539.
APA Liang WF,Zhao YL,Liu LQ,Wang YC,Li WJ,&Li GB.(2017).Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics.BIOPHYSICAL JOURNAL,113(7),1531-1539.
MLA Liang WF,et al."Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics".BIOPHYSICAL JOURNAL 113.7(2017):1531-1539.
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