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Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening
Yang WG(杨文广); Yu HB(于海波); Li GX(李恭新); Wei FN(魏发南); Wang YC(王越超); Liu LQ(刘连庆)
Source PublicationLab on a Chip
Indexed BySCI
WOS IDWOS:000417107500006
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (Project No. 61475183, 61433017, 61503258 and U1613220),the Instrument Developing project of the Chinese Academy of Science. (Grant No. YZ201649),the CAS FEA International Partnership Program for creative Research Team. and the Youth Innovation promotion Association CAS.
AbstractCells are frequently studied because they are basic structural, functional, and biological units of living organisms. Extracting features from cellular behaviors can facilitate decision making in medical diagnoses and represents an important aspect in the development of biomedical engineering. Previous studies have just focused on either the individual cell or cell clusters separately, which leads to a great lack of information. Microwell technologies could address the challenges of in vitro cellular studies, from individual cell studies to 3D functional assays, by providing more information from smaller sample volumes and enabling the incorporation of low-cost high-throughput assays in the drug discovery process. To this end, the present study describes an easy-to-use method for fabricating a versatile microwell chip that utilizes a digital micro-mirror device printing system, and the chip can be employed in multidimensional cellular analysis, ranging from the single cell to the 3D spheroid level. The microwell manufacturing process, using a digital mask in place of a conventional physical mask, is based on shadowed light and is full of flexibility. Three different dimensions (single cell (1D), cell monolayer (2D) and cell spheroid (3D)) of one cell type can be formed using a microwell array and the analyses of biological characteristics are achieved separately. Single cells and cell clusters can be controlled via customized geometries of microfabricated selectively adhesive poly(ethylene glycol) diacrylate (PEGDA) wells. The effects of shape on cellular growth and hybrid tissue layers were investigated by peeling off the microwells. Furthermore, 3D multicellular spheroids were successfully established in a controllable and high-throughput format. Preclinical drug screening was investigated and distinct differences were observed in the tolerance response to drug testing between the 2D and 3D conditions. The study results further demonstrate that the high-density microwell chip is an easy-to-use multidimensional cellular analysis and rapid drug screening technique, and it could be easily adapted for a wide range of biological research applications.
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorYu HB(于海波); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110000, P. R. China
2.University of Chinese Academy of Sciences, Beijing 100049, P. R. China
3.School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350000, P. R. China
Recommended Citation
GB/T 7714
Yang WG,Yu HB,Li GX,et al. Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening[J]. Lab on a Chip,2017,17(24):4243-4252.
APA Yang WG,Yu HB,Li GX,Wei FN,Wang YC,&Liu LQ.(2017).Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening.Lab on a Chip,17(24),4243-4252.
MLA Yang WG,et al."Mask-free fabrication of a versatile microwell chip for multidimensional cellular analysis and drug screening".Lab on a Chip 17.24(2017):4243-4252.
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